Daily variance of urinary excretion of AQP2 determined by sandwich ELISA method
| Autor: | Masahiro Muraguchi, Yasukazu Ohmoto, Fusako Iwata, Sei Sasaki, Toyoki Mori |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Nephrology
medicine.medical_specialty Vasopressin Physiology Urology Enzyme-Linked Immunosorbent Assay Urine urologic and male genital diseases Sensitivity and Specificity Urinary excretion Physiology (medical) Internal medicine Humans Medicine Kidney Tubules Collecting Elisa method Kidney Aquaporin 2 urogenital system business.industry Circadian Rhythm Endocrinology medicine.anatomical_structure business Water balance disorders |
| Zdroj: | Clinical and Experimental Nephrology. 16:406-410 |
| ISSN: | 1437-7799 1342-1751 |
| DOI: | 10.1007/s10157-011-0574-2 |
| Popis: | Urinary excretion of aquaporin 2 (AQP2) is a useful marker of kidney collecting duct function. A specific and convenient method to measure AQP2 in human urine would help to treat water balance disorders. It is unknown whether urinary excretion of AQP2 shows any daily variance.A sandwich enzyme-linked immunosorbent assay (ELISA) method for AQP2 was established using two different kinds of antibodies, and its sensitivity and specificity were examined. Daily variance of urinary excretion of AQP2 and responses to acute water load were examined.The established ELISA specifically detected urine AQP2 with high sensitivity (detected as low as 0.34 pmol/mL). Urinary excretion of AQP2 did not show daily variance between 9 a.m. and 9 p.m. in healthy subjects.The developed ELISA method using two different antibodies is convenient and highly sensitive, and could be useful in clinical practice. Urinary excretion of AQP2 is relatively constant from morning to night, and spot urine sampling at any time during this time period does not affect the results. |
| Databáze: | OpenAIRE |
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