Effects of synthetic pyrethroids on RTG-2 cells
| Autor: | Ayhan Filazi, H. Mehtap Kutlu, Figen Erkoç, Djanan Vejselova, Begum Yurdakok-Dikmen |
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| Přispěvatelé: | Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Kutlu, Hatice Mehtap |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Confocal Microscopy Synthetic Pyrethroids Cytotoxicity Vacuole 010501 environmental sciences Biology Toxicology 01 natural sciences Hydropic degeneration 03 medical and health sciences Mechanism Of Action parasitic diseases medicine Viability assay 0105 earth and related environmental sciences Rtg-2 Anatomy medicine.disease Cell biology 030104 developmental biology medicine.anatomical_structure Cytoplasm Cell culture Micronucleus Nucleus Pyknosis |
| Zdroj: | Toxin Reviews. 37:304-312 |
| ISSN: | 1556-9551 1556-9543 |
| DOI: | 10.1080/15569543.2017.1366922 |
| Popis: | WOS: 000452060300006 The cellular and subcellular effects of some synthetic pyrethroids (permethrin, cypermethrin, tetramethrin, and deltamethrin) on RTG-2 trout cell line were assessed. Mitochondrial function/cell viability and membrane integrity were measured by MTT and LDH release assays. Major morphological changes as evaluated by light and confocal microscopy were treated cells had fragmented morphology and condensed nuclei, fragmentations with hole formation in cytoskeleton, pyknotic nuclei and micronucleus formation, shrinkage of the cells and nuclei. Generation of cytoplasmic vacuoles, prominent induction of cellular pleomorphism, and hydropic degeneration were recorded as compared with controls showing fusiform cells and central nucleus by H&E staining. |
| Databáze: | OpenAIRE |
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