Tombusvirus-based vector systems to permit over-expression of genes or that serve as sensors of antiviral RNA silencing in plants
| Autor: | Rustem T. Omarov, Maria R. Mendoza, John A. Lindbo, Malika Shamekova, Herman B. Scholthof, Yi-Cheng Hsieh |
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| Rok vydání: | 2014 |
| Předmět: |
Agroinfiltration
Tombusvirus Genetic Vectors Green Fluorescent Proteins Gene Expression Nicotiana benthamiana Biology Virology Gene expression Gene vector Gene silencing Gene Silencing Transgenes Antiviral RNA silencing Plant Proteins Suppression TBSV fungi Gene Transfer Techniques food and beverages RNA Plants Plants Genetically Modified biology.organism_classification Virus RNA silencing Tomato bushy stunt virus |
| Zdroj: | Virology. :159-165 |
| ISSN: | 0042-6822 |
| Popis: | A next generation Tomato bushy stunt virus (TBSV) coat protein gene replacement vector system is described that can be applied by either RNA inoculation or through agroinfiltration. A vector expressing GFP rapidly yields high levels of transient gene expression in inoculated leaves of various plant species, as illustrated for Nicotiana benthamiana, cowpea, tomato, pepper, and lettuce. A start-codon mutation to down-regulate the dose of the P19 silencing suppressor reduces GFP accumulation, whereas mutations that result in undetectable levels of P19 trigger rapid silencing of GFP. Compared to existing virus vectors the TBSV system has a unique combination of a very broad host range, rapid and high levels of replication and gene expression, and the ability to regulate its suppressor. These features are attractive for quick transient assays in numerous plant species for over-expression of genes of interest, or as a sensor to monitor the efficacy of antiviral RNA silencing. |
| Databáze: | OpenAIRE |
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