A p53-Responsive miRNA Network Promotes Cancer Cell Quiescence
| Autor: | Simonne Sherwin, Yuan Yuan Zhang, Tao Liu, Rick F. Thorne, Nicole Cole, Su Tang Guo, Xu Guang Yan, Guang Zhi Liu, Yu Chen Feng, Xudong Zhang, Hessam Tabatabaee, Margaret Farrelly, Hamed Yari, Ting La, Lei Jin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Population Apoptosis Biology Models Biological Mice 03 medical and health sciences Cell quiescence Genes Reporter RNA interference Cell Line Tumor Neoplasms microRNA medicine Animals Humans Gene Regulatory Networks Phosphorylation education S-Phase Kinase-Associated Proteins Cellular Senescence Regulation of gene expression education.field_of_study Kinase Cell Cycle Cancer medicine.disease Cell biology Gene Expression Regulation Neoplastic Genes cdc MicroRNAs 030104 developmental biology Oncology Cancer cell RNA Interference Tumor Suppressor Protein p53 |
| Zdroj: | Cancer Research. 78:6666-6679 |
| ISSN: | 1538-7445 0008-5472 |
| Popis: | Cancer cells in quiescence (G0 phase) are resistant to death, and re-entry of quiescent cancer cells into the cell-cycle plays an important role in cancer recurrence. Here we show that two p53-responsive miRNAs utilize distinct but complementary mechanisms to promote cancer cell quiescence by facilitating stabilization of p27. Purified quiescent B16 mouse melanoma cells expressed higher levels of miRNA-27b-3p and miRNA-455-3p relative to their proliferating counterparts. Induction of quiescence resulted in increased levels of these miRNAs in diverse types of human cancer cell lines. Inhibition of miRNA-27b-3p or miRNA-455-3p reduced, whereas its overexpression increased, the proportion of quiescent cells in the population, indicating that these miRNAs promote cancer cell quiescence. Accordingly, cancer xenografts bearing miRNA-27b-3p or miRNA-455-3p mimics were retarded in growth. miRNA-27b-3p targeted cyclin-dependent kinase regulatory subunit 1 (CKS1B), leading to reduction in p27 polyubiquitination mediated by S-phase kinase-associated protein 2 (Skp2). miRNA-455-3p targeted CDK2-associated cullin domain 1 (CAC1), which enhanced CDK2-mediated phosphorylation of p27 necessary for its polyubiquitination. Of note, the gene encoding miRNA-27b-3p was embedded in the intron of the chromosome 9 open reading frame 3 gene that was transcriptionally activated by p53. Similarly, the host gene of miRNA-455-3p, collagen alpha-1 (XXVII) chain, was also a p53 transcriptional target. Collectively, our results identify miRNA-27b-3p and miRNA-455-3p as important regulators of cancer cell quiescence in response to p53 and suggest that manipulating miRNA-27b-3p and miRNA-455-3p may constitute novel therapeutic avenues for improving outcomes of cancer treatment. Significance: Two novel p53-responsive microRNAs whose distinct mechanisms of action both stabilize p27 to promote cell quiescence and may serve as therapeutic avenues for improving outcomes of cancer treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|