Increased Expression of Endothelial and Neuronal Nitric Oxide Synthase In Dura and Pia Mater After Air Stress

Autor: Inger Jansen-Olesen, R Illum, T Zinck
Rok vydání: 2006
Předmět:
Male
Dura mater
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type I
Pathogenesis
chemistry.chemical_compound
0302 clinical medicine
Enos
Vasoconstrictor Agents
030212 general & internal medicine
Air Movements
integumentary system
biology
Pia mater
Reverse Transcriptase Polymerase Chain Reaction
Sumatriptan
General Medicine
Anatomy
musculoskeletal system
Pathophysiology
Nitric oxide synthase
medicine.anatomical_structure
Pia Mater
musculoskeletal diseases
medicine.medical_specialty
Nitric Oxide Synthase Type III
Migraine Disorders
Blotting
Western
Nitric Oxide
Gene Expression Regulation
Enzymologic
Nitric oxide
03 medical and health sciences
Stress
Physiological
Internal medicine
medicine
Animals
RNA
Messenger
Rats
Wistar
business.industry
Meninges
biology.organism_classification
Rats
Endocrinology
nervous system
chemistry
biology.protein
Dura Mater
Neurology (clinical)
Corticosterone
business
030217 neurology & neurosurgery
Zdroj: Cephalalgia. 26:14-25
ISSN: 1468-2982
0333-1024
DOI: 10.1111/j.1468-2982.2005.00978.x
Popis: Stress is the leading precipitating factor for migraine attacks but the underlying mechanism is currently unknown. Nitric oxide (NO) has been implicated in migraine pathogenesis based on the ability of NO donors to induce migraine attacks. In the present study, we investigated in Wistar rats the effect of air stress on nitric oxide synthase (NOS) mRNA and protein expression in dura and pia mater using real-time polymerase chain reaction and Western blotting, respectively. Endothelial (e)NOS protein expression was significantly increased in dura and pia mater after air stress. Significantly augmented neuronal (n)NOS protein expression was detected in pia mater after air stress but not in dura mater. Inducible NOS mRNA and protein expression levels in dura and pia mater were unaffected by stress. The increased expression of eNOS in dura mater and eNOS and nNOS in pia mater seen after stress could not be antagonized by treatment with the migraine drug sumatriptan. These findings point towards the involvement of increased NO concentrations in dura and pia mater in response to air stress. However, the role of these findings in relation to migraine pathophysiology remains unclear.
Databáze: OpenAIRE
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