Development of a Point-of-Care System Based on White Light Reflectance Spectroscopy: Application in CRP Determination
| Autor: | Georgios Koukouvinos, Zoi Ekaterinidi, Panagiota S. Petrou, Vasilios Christianidis, Konstantina Panagiotopoulou, Dimitra Tsounidi, Ioannis Raptis, Evangelia Legaki, Vasiliki Giogli, Sotirios E. Kakabakos, Styliani Taka |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Light Reflectance spectroscopy Point-of-Care Systems Clinical Biochemistry 02 engineering and technology Normal values Biosensing Techniques point-of care 01 natural sciences Article Antibodies Limit of Detection label-free detection White light white light reflectance spectroscopy Humans Diagnostic laboratory Point of care Chromatography Capture antibody Dynamic range Spectrum Analysis 010401 analytical chemistry General Medicine Equipment Design C-Reactive protein 021001 nanoscience & nanotechnology 0104 chemical sciences Human plasma 0210 nano-technology TP248.13-248.65 Biotechnology |
| Zdroj: | Biosensors Volume 11 Issue 8 Biosensors, Vol 11, Iss 268, p 268 (2021) |
| ISSN: | 2079-6374 |
| DOI: | 10.3390/bios11080268 |
| Popis: | The development of methods and miniaturized systems for fast and reliable quantitative determinations at the Point-of-Care is a top challenge and priority in diagnostics. In this work, a compact bench-top system, based on White Light Reflectance Spectroscopy, is introduced and evaluated in an application with high clinical interest, namely the determination of C-Reactive protein (CRP) in human blood samples. The system encompassed all the necessary electronic and optical components for the performance of the assay, while the dedicated software provided the sequence and duration of assay steps, the reagents flow rate, the real-time monitoring of sensor response, and data processing to deliver in short time and accurately the CPR concentration in the sample. The CRP assay included two steps, the first comprising the binding of sample CRP onto the chip immobilized capture antibody and the second the reaction of the surface immunosorbed CRP molecules with the detection antibody. The assay duration was 12 min and the dynamic range was from 0.05 to 200 μg/mL, covering both normal values and acute inflammation incidents. There was an excellent agreement between CRP values determined in human plasma samples using the developed device with those received for the same samples by a standard diagnostic laboratory method. |
| Databáze: | OpenAIRE |
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