Comparative deep transcriptional profiling of four developing oilseeds
| Autor: | Xia Cao, Markus Pauly, Aruna Kilaru, Jacob Krüger Jensen, Jilian Fan, John B. Ohlrogge, Nicholas Thrower, Timothy P. Durrett, Curtis G. Wilkerson, Manuel Adrián Troncoso-Ponce |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
fatty acid biosynthesis Acylation Gene Expression Plant Science Computational biology Biology Genes Plant 01 natural sciences Transcriptome 03 medical and health sciences Tropaeolum comparative transcriptomics Gene Expression Regulation Plant Gene expression lipid metabolism Pyruvic Acid Genetics Plant Oils Gene Triglycerides 030304 developmental biology 2. Zero hunger Regulation of gene expression Expressed Sequence Tags 0303 health sciences Expressed sequence tag triacylglycerol synthesis Ricinus Gene Expression Profiling Brassica napus Euonymus Fatty Acids food and beverages Cell Biology Original Articles Gene expression profiling pyrosequencing Biochemistry Seeds Pyrosequencing Functional genomics Glycolysis Acyltransferases 010606 plant biology & botany |
| Zdroj: | The Plant Journal |
| ISSN: | 1365-313X 0960-7412 |
| Popis: | Transcriptome analysis based on deep expressed sequence tag (EST) sequencing allows quantitative comparisons of gene expression across multiple species. Using pyrosequencing, we generated over 7 million ESTs from four stages of developing seeds of Ricinus communis, Brassica napus, Euonymus alatus and Tropaeolum majus, which differ in their storage tissue for oil, their ability to photosynthesize and in the structure and content of their triacylglycerols (TAG). The larger number of ESTs in these 16 datasets provided reliable estimates of the expression of acyltransferases and other enzymes expressed at low levels. Analysis of EST levels from these oilseeds revealed both conserved and distinct species-specific expression patterns for genes involved in the synthesis of glycerolipids and their precursors. Independent of the species and tissue type, ESTs for core fatty acid synthesis enzymes maintained a conserved stoichiometry and a strong correlation in temporal profiles throughout seed development. However, ESTs associated with non-plastid enzymes of oil biosynthesis displayed dissimilar temporal patterns indicative of different regulation. The EST levels for several genes potentially involved in accumulation of unusual TAG structures were distinct. Comparison of expression of members from multi-gene families allowed the identification of specific isoforms with conserved function in oil biosynthesis. In all four oilseeds, ESTs for Rubisco were present, suggesting its possible role in carbon metabolism, irrespective of light availability. Together, these data provide a resource for use in comparative and functional genomics of diverse oilseeds. Expression data for more than 350 genes encoding enzymes and proteins involved in lipid metabolism are available at the 'ARALIP' website (http://aralip.plantbiology.msu.edu/). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text