Molecular, Biochemical and Ultrastructural Changes Induced by Pb Toxicity in Seedlings of Theobroma cacao L
| Autor: | Alex-Alan Furtado de Almeida, Carlos Priminho Pirovani, Pedro Antônio Oliveira Mangabeira, Graciele Santos Monteiro Reis, Andressa Vieira de Castro, Nicolle Moreira de Almeida |
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| Rok vydání: | 2014 |
| Předmět: |
Chloroplasts
Thiobarbituric acid lcsh:Medicine Self-Fertilization Plant Roots Lipid peroxidation chemistry.chemical_compound Gene Expression Regulation Plant Botany Phytochelatins Soil Pollutants lcsh:Science Peroxidase chemistry.chemical_classification Reactive oxygen species Cacao Multidisciplinary biology lcsh:R Plant Leaves Oxidative Stress chemistry Biochemistry Lead Germination Seedlings Shoot biology.protein lcsh:Q Phytochelatin Guaiacol Lipid Peroxidation Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 7, p e0129696 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Pb is a metal which is highly toxic to plants and animals, including humans. High concentrations of Pb have been observed in beans of T. cacao, as well as in its products. In this work, we evaluated the molecular, biochemical, and ultrastructural alterations in mature leaves and primary roots of seedlings of two progenies of T. cacao, obtained from seed germination in different concentrations of Pb (0, 0.05, 0.1, 0.2, 0.4, 0.8 g L(-1)), in the form of Pb(NO3)2. The progenies resulted from self-fertilization of Catongo and a cross of CCN-10 x SCA-6. The Pb, supplied via seminal, caused alterations in the ultrastructures of the mesophyll cells and in the amount of starch grains in the chloroplasts. The dosage of substances reactive to thiobarbituric acid showed that Pb induced lipid peroxidation. The activity of guaiacol peroxidases and the expression of genes associated to synthetase of phytochelatin, SODcyt and PER increased in response to Pb. In addition, there was alteration in the expression of stress-related proteins. The progeny of CCN-10 x SCA-6 was more tolerant to Pb stress when compared to Catongo, since: (i) it accumulated more Pb in the roots, preventing its translocation to the shoot; (ii) it presented higher activity of peroxidases in the roots, which are enzymes involved in the elimination of excess of reactive oxygen species; and (iii) increased expression of the gene in the phytochelatin biosynthesis route. The results of the proteomic analysis were of paramount importance to differentiate the defense mechanisms used by both progenies of T. cacao. |
| Databáze: | OpenAIRE |
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