Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease
| Autor: | Rosauro Varo, Mariona Bustamante, Mercedes Rubio, Himanshu Gupta, Antonio Sitoe, Pau Cisteró, Xavier Martiáñez-Vendrell, Quique Bassat, Allison Brimacombe, Lorena Pantano, Diana Barrios, Alfredo Mayor, Lola Madrid, Alfons Jiménez, Inocencia Cuamba |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Epidemiology 030231 tropical medicine Plasmodium falciparum malaria lcsh:Medicine Inflammation Disease Biology lcsh:Infectious and parasitic diseases 03 medical and health sciences Histidine-rich protein 2 0302 clinical medicine microRNA parasitic diseases medicine Humans Parasites lcsh:RC109-216 Biomass 030212 general & internal medicine Malaria Falciparum Child Mozambique miRNA Research Organ dysfunction lcsh:R biomarkers Vector-borne infections biology.organism_classification medicine.disease Reverse transcription polymerase chain reaction MicroRNAs Infectious Diseases Spain Immunology severe malaria Biomarker (medicine) next-generation sequencing Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease medicine.symptom Malaria |
| Zdroj: | Emerging Infectious Diseases, Vol 27, Iss 2, Pp 430-442 (2021) Emerging Infectious Diseases |
| ISSN: | 1080-6059 1080-6040 |
| Popis: | Severe malaria (SM) is a major public health problem in malaria-endemic countries. Sequestration of Plasmodium falciparum–infected erythrocytes in vital organs and the associated inflammation leads to organ dysfunction. MicroRNAs (miRNAs), which are rapidly released from damaged tissues into the host fluids, constitute a promising biomarker for the prognosis of SM. We applied next-generation sequencing to evaluate the differential expression of miRNAs in SM and in uncomplicated malaria (UM) in children in Mozambique. Six miRNAs were associated with in vitro P. falciparum cytoadhesion, severity in children, and P. falciparum biomass. Relative expression of hsa-miR-4497 quantified by TaqMan-quantitative reverse transcription PCR was higher in plasma of children with SM than those with UM (p |
| Databáze: | OpenAIRE |
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