Palmitate uptake by neonatal rat myocytes and hepatocytes
| Autor: | Lorrie A. Kirshenbaum, Gu Qi Wang, Bassam M. Elmadhoun, Frank J. Burczynski |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Palmitates
Biology Biochemistry Membrane Potentials Extracellular medicine Animals Myocyte Isoelectric Point Cells Cultured Conalbumin chemistry.chemical_classification Drug Carriers Myocardium Cell Membrane Albumin Biological Transport Serum Albumin Bovine Orosomucoid Ligand (biochemistry) Rats medicine.anatomical_structure Isoelectric point Animals Newborn Liver chemistry Hepatocyte Muramidase Carrier Proteins Extracellular Space Glycoprotein |
| Zdroj: | European Journal of Biochemistry. 268:3145-3153 |
| ISSN: | 0014-2956 |
| DOI: | 10.1046/j.1432-1327.2001.02190.x |
| Popis: | The role of extracellular binding proteins in the rate of [3H]palmitate uptake by neonatal cardiac myocytes and hepatocytes was investigated using a model-independent approach. Binding proteins used in this study included alpha1-acid glycoprotein [isoelectric point (pI) approximately 2.7], conalbumin (pI approximately 6.4), lysozyme (pI approximately 11.0), albumin (pI approximately 4.9), and albumin which had been modified to yield proteins with pI values of 3.5, 4.7, 7.5 and 8.6. All uptake studies were conducted at similar unbound ligand fractions. There was a linear relationship between the rate of neonatal hepatocyte [3H]palmitate clearance and protein pI (r2 = 0.98). In contrast, there was an overall poor relationship between neonatal cardiac myocyte [3H]palmitate-clearance rate and protein pI (r2 = 0.48). However, the relationship improved when the data on [3H]palmitate-clearance were analyzed using only the modified albumins. The study indicates that an ionic interaction between extracellular proteins and the hepatocyte surface enhances the overall uptake of [3H]palmitate. This interaction may be limited to albumin for neonatal cardiac myocytes. |
| Databáze: | OpenAIRE |
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