A yeast FRET biosensor enlightens cAMP signaling
| Autor: | Johan H. van Heerden, Frank J. Bruggeman, Tom O'Toole, Bas Teusink, Joachim Goedhart, Dennis Botman |
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| Přispěvatelé: | VU University medical center, AIMMS, Systems Bioinformatics |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Biosensing Techniques Saccharomyces cerevisiae Biology Flow cytometry CAMP signaling Cyclic AMP Fluorescence Resonance Energy Transfer medicine Extracellular Prospective Studies Molecular Biology medicine.diagnostic_test Articles Cell Biology Metabolism Flow Cytometry Cyclic AMP-Dependent Protein Kinases Yeast High-Throughput Screening Assays Glucose Förster resonance energy transfer Biophysics Single-Cell Analysis Biosensor Intracellular Signal Transduction |
| Zdroj: | Botman, D, O'Toole, T G, Goedhart, J, Bruggeman, F J, van Heerden, J H & Teusink, B 2021, ' A yeast FRET biosensor enlightens cAMP signaling ', Molecular Biology of the Cell, vol. 32, no. 13, pp. 1229-1240 . https://doi.org/10.1091/mbc.E20-05-0319 Molecular Biology of the Cell, 32(13), 1229-1240. American Society for Cell Biology Botman, D, O'Toole, T G, Goedhart, J, Bruggeman, F J, van Heerden, J H & Teusink, B 2021, ' A yeast FRET biosensor enlightens cAMP signaling ', Molecular Biology of the Cell, vol. 32, no. 13, pp. 1229-1240 . https://doi.org/10.1091/MBC.E20-05-0319 Molecular Biology of the Cell |
| ISSN: | 1059-1524 |
| DOI: | 10.1091/mbc.E20-05-0319 |
| Popis: | The cAMP-PKA signaling cascade in budding yeast regulates adaptation to changing environments. We developed yEPAC, a FRET-based biosensor for cAMP measurements in yeast. We used this sensor with flow cytometry for high-throughput single cell-level quantification during dynamic changes in response to sudden nutrient transitions. We found that the characteristic cAMP peak differentiates between different carbon source transitions and is rather homogenous among single cells, especially for transitions to glucose. The peaks are mediated by a combination of extracellular sensing and intracellular metabolism. Moreover, the cAMP peak follows the Weber-Fechner law; its height scales with the relative, and not the absolute, change in glucose. Last, our results suggest that the cAMP peak height conveys information about prospective growth rates. In conclusion, our yEPAC-sensor makes possible new avenues for understanding yeast physiology, signaling, and metabolic adaptation. |
| Databáze: | OpenAIRE |
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