Evaluation of the bilateral cardiac afferent distribution at the spinal and vagal ganglia by retrograde labeling
| Autor: | Gürkan Öztürk, Esra Cagavi, Mehmet Serif Aydin, T. Akgul Caglar, M.U. Turhan, Z.B. Durdu, Mehmet Yalçın Günal |
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| Přispěvatelé: | ALKÜ, 0-belirlenecek |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Sensory Receptor Cells Population TRPV1 Sensory system Biology 03 medical and health sciences Mice 0302 clinical medicine Afferent Ganglia Spinal medicine Distribution (pharmacology) Animals Neurons Afferent education Mouse heart Molecular Biology Fluorescent Dyes Retrograde labeling Di-8-ANEPPQ education.field_of_study Mice Inbred BALB C Staining and Labeling General Neuroscience Cardiac Sensory Neurons Nociceptors Heart Vagus Nerve Retrograde Labeling 030104 developmental biology medicine.anatomical_structure Mapping Nociceptor Soma Female Ganglia Nodose Ganglion Cardiac sensory neurons Neurology (clinical) Neuron Afferents Neuroscience 030217 neurology & neurosurgery Mouse Heart Developmental Biology |
| Popis: | gunal, mehmet yalcin/0000-0001-7702-2441; Cagavi, Esra/0000-0002-7199-583X; Turhan, Mehmet Ugurcan/0000-0002-6219-124X WOS: 000600598100022 PubMed: 33171152 The identity of sensory neurons innervating the heart tissue and the extent of information reported to the brain via these neurons are poorly understood. In order to evaluate the multidimensional distribution and abundance of the cardiac spinal and vagal afferents, we assessed the retrograde labeling efficiency of various tracers, and mapped the cardiac afferents qualitatively and quantitatively at the bilateral nodose ganglia (NGs) and dorsal root ganglia (DRGs). From the five different retrograde tracers evaluated, Di-8-ANEPPQ yielded reproducibly the highest labeling efficiency of cardiac afferents. We demonstrated specific cardiac afferents at NGs and C4 to T11 DRG segments. Next, the 2D reconstruction of the tissue sections and 3D imaging of the whole NGs and DRGs revealed homogeneous and bilateral distribution of cardiac afferents. The quantitative analyses of the labeled cardiac afferents demonstrated approximately 5-6% of the soma in NGs that were equally distributed bilaterally. The neuronal character of Di-8-ANEPPQ labeled cells were validated by coimmunostaning with pan-neuronal marker Tuj-1. In addition, the cell diameters of labeled cardiac sensory neurons were found smaller than 20 mu m, implying the nociceptor phenotype confirmed by co-labeling with TRPV1 and Di-8-ANEPPQ. Importantly, co-labeling with two distinct tracers Di-8-ANEPPQ and WGA-647 demonstrated exclusively the same cardiac afferents in DRGs and NGs, validating our findings. Collectively, our findings revealed the cardiac afferents in NGs bilaterally and DRGs with the highest labeling efficiency reported, spatial distribution and quantitation at both 2D and 3D levels, furthering our understanding of this novel neuron population. Scientific and Technological Research Council of Turkey (TUBITAK) under 1001 Scientific and Technological Research Projects Funding ProgramTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115S381]; Istanbul Medipol University [BAP 2018/19] We would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK) under 1001 Scientific and Technological Research Projects Funding Program by project number 115S381 and Istanbul Medipol University scientific research project grant BAP 2018/19. We thank Dr. Deniz Atasoy for rAAV2-retro-tdtomato virus, REMER-SABITA personnel for their technical help and our animal facility MEDITAM personnel for their kind assistance. |
| Databáze: | OpenAIRE |
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