Development of nuclear DNA markers to characterize genetically diverse groups of Misgurnus anguillicaudatus and its closely related species
| Autor: | Michiko Okubo-Murata, Kohei Nakaya, Yukihiro Kodo, Taiju Saito, Michiko Inaba, Masamichi Kuroda, Katsutoshi Arai, Takafumi Fujimoto, Masaru Murakami, Aya Yamada, Kazuto Ninomiya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cobitidae Genetics biology Misgurnus Aquatic Science biology.organism_classification 010603 evolutionary biology 01 natural sciences Genome Repetitive DNA sequence Hybrid Nuclear DNA 03 medical and health sciences genomic DNA 030104 developmental biology RFLP Restriction fragment length polymorphism Primer (molecular biology) Repeated sequence Species identification |
| Zdroj: | Fisheries science. 83(5):743-756 |
| ISSN: | 0919-9268 |
| Popis: | Repetitive DNA sequences, ManDra and ManBgl, were isolated from the DraI and BglII digests of the genomic DNA of Misgurnus anguillicaudatus, respectively. A primer set of ManDra distinguished two genetically different groups (A and B) of M. anguillicaudatus by specific electrophoretograms. A primer set of ManBgl amplified the DNA of M. anguillicaudatus and M. mizolepis. The individuals of M. anguillicaudatus were divided into two groups depending on the fragment sizes, in which the groups A and B (B-1 and B-2) showed 400 and 460 bp, respectively. M. mizolepis was distinguished by a different pattern (400-, 460-, and 510-bp fragments). PCR–RFLP analyses of recombination activating gene 1 gave a clear difference between A or B-2 (443-bp fragment) and B-1 groups (296- and 147-bp fragments). Clonal lineages and hybrids between B-1 and B-2 groups could be identified by appearance of three fragments (443, 296, and 147 bp). The combined analyses using the above three nuclear markers discriminated among nuclear genomes of genetic groups (A, B-1 and B-2) of M. anguillicaudatus and M. mizolepis. In several localities, natural hybridizations between the group B-1 and B-2 loaches and introgressions of clonal mitochondrial genomes into the group B-1 loaches were detected. |
| Databáze: | OpenAIRE |
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