Role of toll-like receptor 4 antagonist Lipopolysaccharide-Rhodobacter sphaeroides on acute stress-induced voluntary ethanol preference and drinking behaviour: In vivo Swiss Albino mouse model
Autor: | Zuraidah Abdullah, Huei Gau Chuang, Muzaimi Mustapha, Sangu Muthuraju, Jafri Malin Abdullah, Zamzuri Idris, NurNaznee Hirni Abd Aziz, Jia Hui Wong, Aziza Alrafiah |
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Rok vydání: | 2021 |
Předmět: |
Lipopolysaccharides
medicine.medical_specialty Alcohol Drinking medicine.drug_class Hippocampus Rhodobacter sphaeroides Biology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Opioid receptor Internal medicine Dopamine receptor D2 medicine Animals Pharmacology (medical) Cyclic adenosine monophosphate Prefrontal cortex Biological Psychiatry Pharmacology Ethanol Antagonist 030227 psychiatry Motor coordination Toll-Like Receptor 4 Psychiatry and Mental health Endocrinology Neurology chemistry TLR4 Neurology (clinical) 030217 neurology & neurosurgery |
Zdroj: | European Neuropsychopharmacology. 45:59-72 |
ISSN: | 0924-977X |
Popis: | The present study focused on investigating the effect of toll-like receptor 4 (TLR4) antagonist Lipopolysaccharide-Rhodobacter sphaeroides(LPS-RS) on acute, stress-induced voluntary ethanol preference and drinking behaviour, neuronal components activation, and gene expression associated with stress and addictive behaviour. This study involved the exposure of restraint stress and social isolation using Swiss Albino mice. Two-bottle choice ethanol preference analysis was used in the evaluation of voluntary ethanol seeking and drinking behaviour. Several behavioural assessments were carried out to assess fear and anxiety-like behaviour, neuromuscular ability, motor coordination and locomotion. Morphological and immunoreactivity analysis and gene expression analysis were done after the completion of behavioural assessments. TLR4 antagonist LPS-RS treated stressed-mice showed a significant decrease in ethanol drinking compared with stressed mice. Behavioural results showed that stress exposure induced fear and anxiety-like behaviour; however; no significant deficit was found on motor coordination, neuromuscular ability, locomotion and exploratory behaviour among groups. Morphological analysis showed no significant change in the prefrontal cortex and hippocampus among all groups, while immunoreactivity analysis showed higher expression of c-Fos in prefrontal cortex and hippocampus, higher TLR4 expression in the prefrontal cortex and glial fibrillary acidic protein (GFAP) in hippocampus among stressed-animals. Stressed-mice also showed significant increase in TLR4, Nuclear Factor-Kappa B (NF-kB), inducible nitric oxide synthase (iNOS), dopamine receptor D2 (DRD2), cyclic adenosine monophosphate (cAMP) response element binding protein-1 (CREB-1) and opioid receptor MU-1 (OPRM-1) genes expression compared with control and LPS-RS treated stressed-mice. As a conclusion, the antagonism of TLR4 could provide therapeutic value in the treatment of stress-induced addiction. |
Databáze: | OpenAIRE |
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