| Autor: |
M.V.S. Suryanarayana, S. Sriramachari, P.K. Ramachandran, G. S. Agarwal, B.R. Gandhe, Rajagopalan Vijayaraghavan, M.P. Kaushik, Kodumudi S. Venkateswaran, S.K. Shinde, Natarajan Gopalan |
| Rok vydání: |
1988 |
| Předmět: |
|
| Zdroj: |
Journal of Chromatography B: Biomedical Sciences and Applications. 426:239-247 |
| ISSN: |
0378-4347 |
| DOI: |
10.1016/s0378-4347(00)81952-0 |
| Popis: |
Carbamylation of the N-terminal valine of haemoglobin with methyl isocyanate in rats and rabbits has been demonstrated in vitro and in vivo by gas chromatography. N-Methylcarbamylated haemoglobin, converted by cyclization into 3-methyl-5-isopropylhydantoin, has been quantified by gas chromatography. Standard hydantoin was synthesized, chemically characterized and used for calibration. The method is simple and reliable in the concentration range 0.06–2 nmol. Carbamylation of haemoglobin by methyl isocyanate in vivo in rats can be identified only above a dose of 1.05 mg/l in inhalation exposures. It is inferred that methyl isocyanate in the “active” form crosses the alveolar and erythrocyte membranes and carbamylates the haemoglobin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
