The leukocyte immunoglobulin-like receptor gp49B1, coexpressed with c-Kit, modulates hematopoiesis and B cell leukemia development
| Autor: | Yizhou Zou, Huiyun Peng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Biophysics Biology Biochemistry Mice 03 medical and health sciences 0302 clinical medicine LILRB2 Leukemia B-Cell medicine Animals Receptors Immunologic Receptor Molecular Biology Mice Knockout Membrane Glycoproteins Cell Biology medicine.disease Hematopoiesis Mice Inbred C57BL Proto-Oncogene Proteins c-kit Leukemia Haematopoiesis 030104 developmental biology 030220 oncology & carcinogenesis B-cell leukemia Cancer cell biology.protein Cancer research Female Antibody Stem cell |
| Zdroj: | Biochemical and Biophysical Research Communications. 565:72-78 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2021.05.090 |
| Popis: | A better understanding of cell-intrinsic factors involved in regulating stem cells and cancer cells will help advance stem cell applications and cancer cell treatment. Previously, we showed that leukocyte immunoglobulin-like receptor B2 (LILRB2) and its mouse ortholog, paired immunoglobulin-like receptor B (PIRB), promote blood stem cell and leukemia development. Another unique mouse paralog to PIRB called gp49B1 was also discovered. However, the roles of gp49B1 in hematopoietic stem cells and leukemia development are largely unknown. Here, we found that gp49B1 is expressed on LSK cells of mouse neonatal hematopoietic organs and is positively correlated with c-Kit expression. However, in noncompetitive and competitive repopulation assays, neonatal splenic gp49B1-positive and c-Kit-highly expressed LSK cells exhibited poor engraftment potential and lymphoid lineage bias. Moreover, in a mouse N-Myc-induced precursor B-acute lymphoblastic leukemia (pre-B ALL) model, we found that gp49B1 deficiency or low levels of c-Kit led to a delay in leukemia development. Together, our results suggest that gp49B1 expressed on hematopoietic progenitor cells supports hematopoietic and leukemia development. |
| Databáze: | OpenAIRE |
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