Comparison of the phagocytic response of rat and hamster alveolar macrophages to man-made vitreous fibers in vitro

Autor: Münzing S, Anne-Marie Allmeling, Fritz Krombach, Martina Dörger
Rok vydání: 2001
Předmět:
0301 basic medicine
Pathology
medicine.medical_specialty
Health
Toxicology and Mutagenesis
Phagocytosis
Hamster
Biology
Toxicology
medicine.disease_cause
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Species Specificity
Superoxides
Cricetinae
Image Interpretation
Computer-Assisted
Macrophages
Alveolar
medicine
Macrophage
Animals
Cells
Cultured
chemistry.chemical_classification
Mineral Fibers
Reactive oxygen species
Microscopy
Video
030102 biochemistry & molecular biology
medicine.diagnostic_test
L-Lactate Dehydrogenase
Mesocricetus
Superoxide
General Medicine
Molecular biology
Rats
medicine.anatomical_structure
Bronchoalveolar lavage
chemistry
030220 oncology & carcinogenesis
Pulmonary alveolus
Reactive Oxygen Species
Bronchoalveolar Lavage Fluid
Oxidative stress
Zdroj: Humanexperimental toxicology. 19(11)
ISSN: 0960-3271
Popis: Rats and hamsters are well known for their disparate response to inhaled mineral fibers/particles. Alveolar macrophages (AM) play an important role in the pulmonary clearance and retention of mineral fibers/particles mainly through the process of phagocytosis. The aim of this study was to investigate whether there exist differences in the phagocytic response and release of reactive oxygen species (ROS) between rat and hamster AM upon exposure to man-made vitreous fibers (MMVF) in vitro. AM were obtained by bronchoalveolar lavage and macrophage-enriched cultures were exposed to MMVFlo and MMVF21 fibers for 20 h. The phagocytic response of macrophages was determined by computer-assisted video-microscopy and the superoxide anion production was evaluated by cytochrome c reduction. A significantly higher percentage of rat AM underwent frustrated phagocytosis of both types of MMVF compared to hamster AM. This was associated with a higher ROS release by rat AM compared to hamster AM. These data may help to explain the cellular mechanisms underlying the disparate pulmonary response of rat and hamster to inhaled particulate matter.
Databáze: OpenAIRE
Externí odkaz: