Profiling of Saccharomyces cerevisiae transcription factors for engineering the resistance of yeast to lignocellulose-derived inhibitors in biomass conversion
| Autor: | Guochao Wu, Zixiang Xu, Leif J. Jönsson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
BIOTECHNOLOGY AND BIOENGINEERING P1307 reira Clara MICROBIAL CELL FACTORIES lcsh:QR1-502 JOURNAL OF BIOLOGICAL CHEMISTRY V66 Multidrug resistance Applied Microbiology and Biotechnology Lignin lcsh:Microbiology MOLECULAR & GENERAL GENETICS V29 chemistry.chemical_compound Transcription ctors Biomass Acrolein V244 Sequence Deletion YAP1 STB5 VOL 73 Biochemistry and Molecular Biology olenice FEMS YEAST RESEARCH2nd International Meeting on Yeast Apoptosis SEP Coniferyl aldehyde Biochemistry P1933 vka Adnan Genetic Engineering Lignocellulosic biomass conversion Biotechnology P571 innen Steve PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Bioengineering Biology P133 lshan Kailash 03 medical and health sciences P52641 i Z EUKARYOTIC CELL Drug Resistance Multiple Fungal V108 Transcription factors Furaldehyde mougrand N Gene Transcription factor Research P501 cau-Danila A biology.organism_classification V6 P2592 ye-Rowley WS V5 Yeast Multiple drug resistance Oxidative Stress 030104 developmental biology chemistry Fermentation V16 MOLECULAR MICROBIOLOGY LAVEAU T SLOVAKIA Biokemi och molekylärbiologi V278 |
| Zdroj: | Microbial Cell Factories, Vol 16, Iss 1, Pp 1-15 (2017) Microbial Cell Factories |
| Popis: | Background Yeast transcription factors (TFs) involved in the regulation of multidrug resistance (MDR) were investigated in experiments with deletion mutants, transformants overexpressing synthetic genes encoding TFs, and toxic concentrations of lignocellulose-derived substances added to cultures as complex mixtures or as specific compounds, viz. coniferyl aldehyde, 5-hydroxymethylfurfural, and furfural. Results In the presence of complex mixtures of toxic substances from spruce wood, transformants overexpressing YAP1 and STB5, TFs involved in oxidative stress response, exhibited enhanced relative growth rates amounting to 4.589 ± 0.261 and 1.455 ± 0.185, respectively. Other TFs identified as important for resistance included DAL81, GZF3, LEU3, PUT3, and WAR1. Potential overlapping functions of YAP1 and STB5 were investigated in experiments with permutations of deletions and overexpression of the two genes. YAP1 complemented STB5 with respect to resistance to 5-hydroxymethylfurfural, but had a distinct role with regard to resistance to coniferyl aldehyde as deletion of YAP1 rendered the cell incapable of resisting coniferyl aldehyde even if STB5 was overexpressed. Conclusions We have investigated 30 deletion mutants and eight transformants overexpressing MDR transcription factors with regard to the roles the transcription factors play in the resistance to toxic concentrations of lignocellulose-derived substances. This work provides an overview of the involvement of thirty transcription factors in the resistance to lignocellulose-derived substances, shows distinct and complementary roles played by YAP1 and STB5, and offers directions for the engineering of robust yeast strains for fermentation processes based on lignocellulosic feedstocks. Electronic supplementary material The online version of this article (10.1186/s12934-017-0811-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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