Evaluation of engraftment by ABO genotypic analysis of erythroid burst-forming units after bone marrow transplantation
| Autor: | Takashi Obata, Yoko Nakagi, Sadahiko Iwamoto, Keiya Ozawa, Takahiro Nagashima, Kiyoshi Watari, Koji Kishino, Naoko Sugano, Kazuo Muroi, Chizuru Kawano |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male congenital hereditary and neonatal diseases and abnormalities Cancer Research Myeloid Erythrocytes Adolescent Genotype Biology Polymerase Chain Reaction ABO Blood-Group System Colony-Forming Units Assay hemic and lymphatic diseases ABO blood group system parasitic diseases medicine Humans Erythropoiesis Bone Marrow Transplantation DNA Primers Erythroid Precursor Cells Graft Survival hemic and immune systems Hematology Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Flow Cytometry biological factors Histocompatibility Red blood cell Leukemia Leukemia Myeloid Acute medicine.anatomical_structure Phenotype Oncology Immunology Leukemia Myeloid Chronic-Phase Bone marrow |
| Zdroj: | Leukemia research. 26(1) |
| ISSN: | 0145-2126 |
| Popis: | Six patients received an allogeneic bone marrow transplant from HLA-identical ABO-mismatched donors. ABO genotype of erythroid burst-forming units (BFU-E) from peripheral blood was analyzed using polymerase chain reaction with sequence specific primers (PCR-SSP). After bone marrow transplantation (BMT), engraftment of donor cells by ABO genotypic analysis of BFU-E was compared with ABO phenotypic analysis of red blood cells (RBCs). During the early stage after BMT, ABO genotype of BFU-E in the recipients converted to that of the donors. In contrast, mixed ABO phenotype of RBCs persisted for about 3 months. In one patient, autologous hemopoietic cell recovery was detected by the ABO genotypic analysis before clinical manifestation. ABO genotypic analysis of BFU-E is relevant for enagraftment after ABO-mismatched BMT. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect