Identification of epigenetic variation associated with synchronous pod maturity in mungbean (Vigna radiata L.)
| Autor: | Hakyung Kwon, Jungmin Ha, Suk-Ha Lee, Moon Young Kim, Min Young Yoon, Kang-Heum Cho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Epigenomics Crops Agricultural lcsh:Medicine Single-nucleotide polymorphism Biology Genes Plant 01 natural sciences Polymorphism Single Nucleotide Article Plant breeding Epigenesis Genetic 03 medical and health sciences Genotype Epigenetics lcsh:Science Gene Genetics Multidisciplinary Vigna lcsh:R fungi Methylation DNA Methylation 030104 developmental biology Differentially methylated regions CpG site DNA methylation lcsh:Q 010606 plant biology & botany |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Cytosine methylation in genomic DNA affects gene expression, potentially causing phenotypic variation. Mungbean, an agronomically and nutritionally important legume species, is characterized by nonsynchronous pod maturity, resulting in multiple harvest which costs extra time and labor. To elucidate the epigenetic influences on synchronous pod maturity (SPM) in mungbean, we determined the genome-wide DNA methylation profiles of eight mungbean recombinant inbred lines (RILs) and their parental genotypes, and compared DNA methylation profiles between high SPM and low SPM RILs, thus revealing differentially methylated regions (DMRs). A total of 3, 18, and 28 pure DMRs, defined as regions showing no significant correlation between nucleotide sequence variation and methylation level, were identified in CpG, CHG, and CHH contexts, respectively. These DMRs were proximal to 20 genes. Among the 544 single nucleotide polymorphisms identified near the 20 genes, only one caused critical change in gene expression by early termination. Analysis of these genome-wide DNA methylation profiles suggests that epigenetic changes can influence the expression of proximal genes, regardless of nucleotide sequence variation, and that SPM is mediated through gibberellin-mediated hormone signaling pathways. These results provide insights into how epialleles contribute to phenotypic variation and improve SPM in mungbean cultivars. |
| Databáze: | OpenAIRE |
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