Activation of 5-HT1A receptors inhibits carbachol-stimulated inositol 1,4,5-trisphosphate mass accumulation in the rodent hippocampus
| Autor: | J. Benavides, Y. Claustre, C. Minisclou |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Agonist medicine.medical_specialty Carbachol medicine.drug_class Stimulation Inositol 1 4 5-Trisphosphate In Vitro Techniques Biology Hippocampal formation Binding Competitive Hippocampus Biochemistry Rats Sprague-Dawley Radioligand Assay Cellular and Molecular Neuroscience Internal medicine Muscarinic acetylcholine receptor polycyclic compounds medicine Animals heterocyclic compounds Inositol phosphate Receptor Cells Cultured Neurons chemistry.chemical_classification 8-Hydroxy-2-(di-n-propylamino)tetralin Membranes Hydrolysis General Medicine Pirenzepine Stimulation Chemical Rats Serotonin Receptor Agonists Endocrinology nervous system chemistry medicine.drug |
| Zdroj: | Neurochemical Research. 20:977-983 |
| ISSN: | 1573-6903 0364-3190 |
| Popis: | We have previously demonstrated that 5-HT1A receptor agonists partially prevent the stimulation by carbachol of [3H]-phosphoinositide hydrolysis in immature rat hippocampal slices. This negative modulation has been investigated further by measuring, using a radioreceptor assay, the mass accumulation of IP3. In hippocampal slices from developing rats and in hippocampal neurons, carbachol enhanced the accumulation of IP3 and this response was partially inhibited by 8-OH-DPAT with a potency compatible with the affinity of this agonist for 5-HT1A receptors. The inhibition of the carbachol response by 8-OH-DPAT was non-competitive in nature and 8-OH-DPAT did not affect the inhibitory potency of pirenzepine. The inhibitory effect of 8-OH-DPAT was maintained after washing the slices preincubated with this compound but was not observed on the carbachol-stimulated PIP2 hydrolysis in hippocampal membranes, suggesting that this compound induces long lasting changes of muscarinic receptors and/or their effector mechanism by an indirect action. |
| Databáze: | OpenAIRE |
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