Successful cryopreservation of purified autologous CD34+ cells: influence of freezing parameters on cell recovery and engraftment
| Autor: | C Rieux, Marine Divine, M Janvier, F. Beaujean, Jose-Luis Pico, J. H. Bourhis, H. Jouault, Ch. Bayle, Ch Le Forestier |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Time Factors Cell Survival CD34 Antigens CD34 In Vitro Techniques Biology Transplantation Autologous Cryopreservation Colony-Forming Units Assay Andrology Cryoprotective Agents Freezing medicine Humans Dimethyl Sulfoxide Progenitor cell Transplantation Graft Survival Hematopoietic Stem Cell Transplantation Hematopoietic stem cell Hematology Middle Aged Hematopoietic Stem Cells Hematopoiesis Haematopoiesis medicine.anatomical_structure Immunology Female Stem cell Ex vivo |
| Zdroj: | Bone Marrow Transplantation. 22:1091-1096 |
| ISSN: | 1476-5365 0268-3369 |
| Popis: | Conventional hematopoietic stem cell cryopreservation methods use a DMSO concentration of 10%. However, cells manipulated ex vivo may require more refined freezing protocols adapted to the specific cell suspension. In this retrospective study, we evaluated the results obtained with CD34+ cells purified from peripheral blood of 39 patients on the CEPRATE SC System and frozen in 7.5% DMSO with a view to transplantation. The post-freezing recovery of progenitor cells was 89.4 +/- 27.87% for CD34+ cells, 59.13 +/- 36.93% for CFU-GM, and 53.49 +/- 40.71 for BFU-E. Neither the purity of the suspension nor the nucleated cell density during freezing was predictive of cell recovery. No difference was observed between cells stored in vials and bags. Thirty-seven patients transplanted with the concentrated CD34+ fraction received 4.46 x 10(6) CD34+ cells/kg and 33.04 x 10(4) CFU-GM/kg. The median time to granulocyte (0.5 x 10(9)/l) and platelet (50 x 10(9)/l) engraftment was 11 and 13 days, respectively. Only cell density and the infused number of CD34+ cells and CFU-GM were significantly related to hematological recovery. Our data suggest that purified CD34+ cells can be successfully cryopreserved in 7.5% DMSO and may represent a first step in establishing freezing parameters for selected CD34+ cells. |
| Databáze: | OpenAIRE |
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