Evaluation of the VITEK 2 Gram Positive (GP) Microbial Identification Test Card: Collaborative Study
| Autor: | Erin, Crowley, Patrick, Bird, Kiel, Fisher, Katherine, Goetz, Megan, Boyle, M Joseph, Benzinger, Marc, Juenger, James, Agin, David, Goins, Ronald L, Johnson |
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| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Journal of AOAC INTERNATIONAL. 95:1425-1432 |
| ISSN: | 1944-7922 1060-3271 |
| Popis: | A collaborative study was conducted to evaluate the performance of the VITEK® 2 Gram Positive (GP) identification card for use with the VITEK 2 automated microbial identification system. The GP test card is used in the identification of selected Gram positive organisms, including Listeria and Staphylococcus species. The VITEK 2 GP card is based on 43 biochemical tests measuring carbon source utilization, inhibition and resistance, and enzymatic activities. A total of 20 laboratories representing government, industry, and private testing laboratories throughout the United States participated. In this study, 720 Gram-positive inclusivity isolates were analyzed by the GP Identification method. Of the 720 well-characterized isolates, 714 were identified correctly, zero were misidentified, zero were unidentified, and six were not characterized as a Gram-positive organism by the VITEK 2 GP method. Additionally, 120 strains exclusive of Gram-positive organisms were screened by Gram stain. A total of 106 isolates were correctly excluded. Fourteen organisms were incorrectly characterized by Gram stain procedures, thus resulting in improper analysis and misidentification by VITEK GP. The VITEK 2 GP identification method is an acceptable automated method for the rapid identification of selected Gram-positive bacteria. |
| Databáze: | OpenAIRE |
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