MicroRNA-21 promotes wound healing via the Smad7-Smad2/3-Elastin pathway
| Autor: | Yitong Liu, Yingying Su, Yongmei Xie, Lijia Guo, Hao Wang, Xiaoyan Li, Juan Du, Songling Wang, Yi Liu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Smad2 Protein Biology Smad7 Protein Extracellular matrix Mice 03 medical and health sciences 0302 clinical medicine Western blot Cell Movement microRNA medicine Animals Humans Smad3 Protein Mice Knockout Wound Healing integumentary system medicine.diagnostic_test Cell Biology Flow Cytometry Elastin Cell biology Staining MicroRNAs 030104 developmental biology Gene Expression Regulation 030220 oncology & carcinogenesis biology.protein Phosphorylation Wound healing Cell signaling pathways Signal Transduction |
| Zdroj: | Experimental Cell Research. 362:245-251 |
| ISSN: | 0014-4827 |
| Popis: | Wound healing is regulated by a complex network of cells, molecules, and cytokines, as well as microRNAs (miRNAs). miRNAs were confirmed to influence the wound healing process, and miR-21, an important member of the miRNA family, was also shown to regulate wound healing. The aim of the present study was to investigate the role of miR-21 in the wound healing process and the possible underlying cell signaling pathways. We isolated GMSCs from WT and miR-21-KO mouse gingiva. Flow cytometric analysis and immunocytofluorescense staining were used to identify the GMSCs acquired from WT and miR-21-KO mice. RT-PCR, western blot analysis and immunohistofluorescence staining were performed to examine the expression of extracellular matrix components and key proteins of cell signaling pathways. TargetScan and pmiR-RB-REPORT vectors were used to verify that Smad7 was a direct target of miR-21. Compared to WT mice, miR-21-KO mice showed slower wound healing. RT-PCR and western blot analysis indicated that Elastin expression was downregulated in miR-21-deficient samples. We confirmed that Smad7 was a direct target of miR-21. miR-21 knockout resulted in increased expression of Smad7 and impaired phosphorylation of the Smad2/3 complex. The expression of the Smad7-Smad2/3-Elastin axis in palate tissues sections acquired from WT and miR-21-KO mice showed the same trend. Based on all these results, we demonstrated that miR-21 promoted the wound healing process via the Smad7-Smad2/3-Elastin pathway. |
| Databáze: | OpenAIRE |
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