Loss of digestive organ expansion factor (Diexf) reveals an essential role during murine embryonic development that is independent of p53
| Autor: | Neeraj K. Aryal, Maurisa Riley-Croce, Guillermina Lozano, Vinod Pant, Amanda R. Wasylishen |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology mouse model Embryogenesis Regulator Anatomy biology.organism_classification Embryonic stem cell Phenotype Cell biology 03 medical and health sciences Def-Capn3 nucleolar pathway 030104 developmental biology Oncology ribosome small subunit processome Knockout mouse Mdm4 co-amplification biology.protein Mdm2 Gene Zebrafish CRISPR/Cas9 Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | Increased levels of inhibitors of the p53 tumor suppressor such as Mdm2 and Mdm4 drive tumor development and thus serve as targets for therapeutic intervention. Recently, digestive organ expansion factor (Diexf) has been identified as a novel inhibitor of p53 in zebrafish. Here, we address the potential role of Diexf as a regulator of the p53 pathway in mammals by generating Diexf knockout mice. We demonstrate that, similar to Mdm2 and Mdm4, homozygous deletion of Diexf is embryonic lethal. However, unlike in Mdm2 and Mdm4 mice, loss of p53 does not rescue this phenotype. Moreover, Diexf heterozygous animals are not sensitive to sub-lethal ionizing radiation. Thus, we conclude that Diexf is an essential developmental gene in the mouse, but is not a significant regulator of the p53 pathway during development or in response to ionizing radiation. |
| Databáze: | OpenAIRE |
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