Toxoplasma gondii activates NLRP12 inflammasome pathway in the BALB/c murine model
| Autor: | Aleksandra Barac, Adel Spotin, Behzad Baradaran, Paria Alizadeh, Yaghob Azadi, Saba Rajabi, Farhad Babaie, Reza Valadan, Mahmoud Mahami-Oskouei, Ehsan Ahmadpour |
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| Rok vydání: | 2021 |
| Předmět: |
Inflammasomes
Veterinary (miscellaneous) Pyrin domain BALB/c 03 medical and health sciences Mice Antigen Western blot Gene expression medicine Animals 030304 developmental biology 0303 health sciences Mice Inbred BALB C biology medicine.diagnostic_test 030306 microbiology Intracellular Signaling Peptides and Proteins Toxoplasma gondii Inflammasome biology.organism_classification medicine.disease Molecular biology Toxoplasmosis 3. Good health Disease Models Animal Infectious Diseases Insect Science Parasitology Toxoplasma medicine.drug |
| Zdroj: | Acta tropica. 225 |
| ISSN: | 1873-6254 |
| Popis: | The host resistance against Toxoplasma gondii (T. gondii) infection is related to the initiation of the immune response. The study aimed to investigate the role of the leucine-rich repeat family, pyrin domain -containing protein 12 (NLRP12), and cytoplasmic nucleotide-binding domain in the inflammasome-mediated cell death during murine toxoplasmosis. Groups of BALB/c mice (n = 10) were inoculated intraperitoneally with live tachyzoites, excretory-secretory antigens (ESAs) of T. gondii RH strain, and RPMI. The gene expression levels of NLRP12, caspase-3, caspase-1, IL-1β, IL-18, ASC, and Bcl-2 were measured in the peritoneal cells using quantitative real-time PCR, while the determination of NLRP12 protein level was measured by Western blot. Also, the intracellular reactive oxygen species (ROS) production was investigated. Quantitative and comparative analyses showed that injection of tachyzoites significantly increased NLRP12, caspase-3, caspase-1, IL-1β, IL-18, and ASC genes mRNA expression levels (p |
| Databáze: | OpenAIRE |
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