Transcriptome profiling and gene expression analyses of eggplant (Solanum melongena L.) under heat stress
| Autor: | Zongwen Zhu, Dingshi Zha, Shengmei Zhang, Aidong Zhang, Haibin Shen, Jing Shang, Xuexia Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Molecular biology Gene Expression RNA-Seq 01 natural sciences Biochemistry Heat Shock Response Antioxidants Transcriptome Sequencing techniques Plant Growth Regulators Gene Expression Regulation Plant Gene expression Heat-Shock Proteins Cellular Stress Responses Plant Proteins Regulation of gene expression Genetics Multidisciplinary biology Physics food and beverages Classical Mechanics Eukaryota RNA sequencing Genomics Plants Experimental Organism Systems Cell Processes RNA Plant Physical Sciences Medicine Mechanical Stress Transcriptome Analysis Research Article Science Arabidopsis Thaliana Brassica Research and Analysis Methods Genes Plant 03 medical and health sciences Model Organisms Plant and Algal Models Heat shock protein DNA-binding proteins Gene Regulation Solanum melongena Gene Biology and life sciences Gene Expression Profiling Organisms Proteins Computational Biology Cell Biology biology.organism_classification Genome Analysis Regulatory Proteins Gene expression profiling 030104 developmental biology Thermal Stresses Molecular biology techniques Animal Studies Solanum Heat-Shock Response 010606 plant biology & botany Transcription Factors |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 8, p e0236980 (2020) |
| ISSN: | 1932-6203 |
| Popis: | Global warming induces heat stress in eggplant, seriously affecting its quality and yield. The response to heat stress is a complex regulatory process; however, the exact mechanism in eggplant is unknown. We analyzed the transcriptome of eggplant under different high-temperature treatments using RNA-Seq technology. Three libraries treated at high temperatures were generated and sequenced. There were 40,733,667, 40,833,852, and 40,301,285 clean reads with 83.98%, 79.69%, and 84.42% of sequences mapped to the eggplant reference genome in groups exposed to 28°C (CK), 38°C (T38), and 43°C (T43), respectively. There were 3,067 and 1,456 DEGs in T38 vs CK and T43 vs CK groups, respectively. In these two DEG groups, 315 and 342 genes were up- and down-regulated, respectively, in common. Differential expression patterns of DEGs in antioxidant enzyme systems, detoxication, phytohormones, and transcription factors under heat stress were investigated. We screened heat stress-related genes for further validation by qRT-PCR. Regulation mechanisms may differ under different temperature treatments, in which heat shock proteins and heat stress transcription factors play vital roles. These results provide insight into the molecular mechanisms of the heat stress response in eggplant and may be useful in crop breeding. |
| Databáze: | OpenAIRE |
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