Compositional and transcriptional analyses of reduced zein kernels derived from the opaque2 mutation and RNAi suppression
| Autor: | Thomas M. Malvar, Meng Wang, Alessandra Frizzi, Wayne E. Brown, James A. Morrell, Shihshieh Huang, Rico A. Caldo, Linda L. Lutfiyya |
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| Rok vydání: | 2009 |
| Předmět: |
genetic structures
Transcription Genetic Zein Lysine Plant Science Biology Zea mays Endosperm RNA interference Gene Expression Regulation Plant Gene expression Genetics Storage protein RNA Messenger Amino Acids Gene Oligonucleotide Array Sequence Analysis Plant Proteins chemistry.chemical_classification Gene Expression Profiling food and beverages RNA Reproducibility of Results General Medicine Blotting Northern Plants Genetically Modified Up-Regulation DNA-Binding Proteins Biochemistry chemistry Plant protein Mutation RNA Interference Agronomy and Crop Science Transcription Factors |
| Zdroj: | Plant molecular biology. 73(4-5) |
| ISSN: | 1573-5028 |
| Popis: | Corn protein is largely made up of a group of nutritionally limited storage proteins known as zein. The reduction of zein can be achieved by a transcriptional mutation, opaque2 (o2), or a transgene targeting zein through RNA interference (RNAi). Zein reduction results in an increase of more nutritionally balanced non-zein proteins, and therefore enhance the overall quality of corn protein. In this study, the composition of mature kernels and the transcriptional profile of developing kernels of these two types of zein reduced kernels were compared. Both zein reduced kernels contained higher levels of lysine and tryptophan and free amino acids were 10–20-folds more abundant than the wild-type counterpart. We also found that free lysine contributed partially to the increased lysine in o2 kernels while protein-bound lysine was mainly responsible for the increased lysine in transgenic zein reduction (TZR) kernels. Although they had relatively similar gene expression patterns in developing endosperm, o2 kernels had greater transcriptional changes than TZR kernels in general. A number of transcripts that were specifically down-regulated in o2 were identified. Many promoter sequences of these transcripts contain putative O2 binding motifs, suggesting that their expression is directly regulated by O2. |
| Databáze: | OpenAIRE |
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