Development of PCR primers for the detection of Salmonella enterica serovar Choleraesuis based on the fliC gene
| Autor: | Wen-Zhe Hwang, Jen-Chieh Pang, Hau-Yang Tsen, Tsai-Hsin Chiu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Serotype
DNA Bacterial Salmonella Food Contamination medicine.disease_cause Microbiology Polymerase Chain Reaction Sensitivity and Specificity law.invention law Multiplex polymerase chain reaction medicine Food microbiology Animals Humans Polymerase chain reaction DNA Primers Salmonella paratyphi C biology Gene Amplification Salmonella enterica biology.organism_classification Enterobacteriaceae Virology Consumer Product Safety Food Microbiology Food Science Flagellin |
| Zdroj: | Journal of food protection. 68(8) |
| ISSN: | 0362-028X |
| Popis: | Salmonella enterica serovar Choleraesuis may cause swine salmonellosis and human infection. Because the conventional method for detection of this Salmonella serovar may take 3 to 5 days, a PCR method for detection was evaluated. By comparing the sequence of the phase 1 flagellin (fliC) gene of Salmonella Choleraesuis with that of other Salmonella serovars and of other bacteria species available in GenBank, two PCR primers (flinC-F and flinC-R) were designed. Using these primers, all 97 Salmonella Choleraesuis strains assayed generated the expected PCR product, with a molecular mass of 963 bp. Except for S. enterica Paratyphi C, Salmonella isolates other than Salmonella Choleraesuis and non-Salmonella isolates, including strains of Enterobacteriaceae, all generated negative PCR results. Salmonella Paratyphi C could be differentiated from Salmonella Choleraesuis through the use of primers designed from the viaB gene. When Salmonella Choleraesuis isolates from swine stool, pork, liver, feed, and human whole blood samples were assayed with a preenrichment step, as low as 1 CFU/g or ml of the original sample could be detected. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|