Recruitment of Staufen2 Enhances Dendritic Localization of an Intron-Containing CaMKIIα mRNA
| Autor: | Maya V. Georgieva, Carme Gallego, Sara Gutiérrez, Raúl Ortiz, Sandra M. Fernández-Moya, Neus Pedraza |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), European Commission |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Stau2 Neurite Dendritic localization Nerve Tissue Proteins Biology General Biochemistry Genetics and Molecular Biology Synaptic plasticity 03 medical and health sciences mRNA transport Mice P-bodies Animals RNA Messenger lcsh:QH301-705.5 Cells Cultured Messenger RNA Intron retention Intron RNA Brain RNA-Binding Proteins Translation (biology) Dendrites Molecular biology Introns Cell biology 030104 developmental biology lcsh:Biology (General) Dendritic transport CaMKIIα Synapses Calcium-Calmodulin-Dependent Protein Kinase Type 2 |
| Zdroj: | Repositorio Abierto de la UdL Universitad de Lleida Digital.CSIC. Repositorio Institucional del CSIC instname Recercat. Dipósit de la Recerca de Catalunya Cell Reports, Vol 20, Iss 1, Pp 13-20 (2017) |
| ISSN: | 2211-1247 |
| Popis: | Regulation of mRNA localization is a conserved cellular process observed in many types of cells and organisms. Asymmetrical mRNA distribution plays a particularly important role in the nervous system, where local translation of localized mRNA represents a key mechanism in synaptic plasticity. CaMKIIα is a very abundant mRNA detected in neurites, consistent with its crucial role at glutamatergic synapses. Here, we report the presence of CaMKIIα mRNA isoforms that contain intron i16 in dendrites, RNA granules, and synaptoneurosomes from primary neurons and brain. This subpopulation of unspliced mRNA preferentially localizes to distal dendrites in a synaptic-activity-dependent manner. Staufen2, a well-established marker of RNA transport in dendrites, interacts with intron i16 sequences and enhances its distal dendritic localization, pointing to the existence of intron-mediated mechanisms in the molecular pathways that modulate dendritic transport and localization of synaptic mRNAs. This work was funded by grants from the Ministry of Economy and Competitiveness of Spain (BFU2014-52591-R CG) and the European Union (FEDER) to C.G. |
| Databáze: | OpenAIRE |
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