Elastases from Human and Canine Granulocytes, I. Some Proteolytic and Esterolytic Properties
| Autor: | Kjell Ohlsson, Inge Olsson, Schiessler H, Magnus Delshammar |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Formates
Granulocyte Biochemistry Dogs Species Specificity Leukocytes medicine Animals Humans Pancreatic elastase chemistry.chemical_classification Granulocyte Elastase Alanine Pancreatic Elastase Esterases Caseins Substrate (chemistry) Elastin Dissociation constant Enzyme medicine.anatomical_structure chemistry alpha 1-Antitrypsin Granulocytes Peptide Hydrolases |
| Zdroj: | Hoppe-Seyler´s Zeitschrift für physiologische Chemie. 357:1245-1250 |
| ISSN: | 0018-4888 |
| Popis: | The lysosome-like granules of human and canine granulocytes contain an enzyme with elastinolytic activity. The enzymatic behaviour of these elastases was further characterized using the protein substrates elastin-orcein and azocasein and the synthetic substrates tert.-butyloxycarbonyl-alanine p-nitrophenylester (Boc-Ala-ONp) and 3-carboxypropionyl-L-alanyl-L-alanyl-L-alanine p-nitroanilide (Suc-Ala3-NHNp) in photometric assays. The affinities of the granulocyte elastases and of porcine pancreatic elastase to these substrates are very similar, e.g. human granulocyte elastase: KM (Boc-Ala-ONp) = 0.35mM, KM (Suc-Ala3-NHNp) = 1.25mM, porcine pancreatic elastase: KM (Boc-Ala-ONp) = 0.3mM, KM (Suc-Ala3-NHNp) - 1.15mM. The most convenient substrate for the assay of human and dog granulocyte elastases and for kinetic measurements with these enzymes is Suc-Ala3-NHNp. Using this substrate, the dissociation constant of the complex of human granulocyte elastase with human alpha1-antitrypsin could be determined (Ki = 3.5 x 10(-10)M). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|