Inflamm-aging and arachadonic acid metabolite differences with stage of tendon disease
| Autor: | Roger Smith, D R E Abayasekara, Natalie Jayne Werling, Jayesh Dudhia, Stephanie G. Dakin, Dirk Werling |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Aging
Anatomy and Physiology medicine.medical_treatment Receptor expression lcsh:Medicine Veterinary Anatomy and Physiology Dinoprost 0403 veterinary science Pathogenesis Tendons chemistry.chemical_compound Animal Musculoskeletal Anatomy Receptors Lipoxin lcsh:Science Connective Tissue Diseases Musculoskeletal System 0303 health sciences Multidisciplinary 04 agricultural and veterinary sciences Lipoxins Veterinary Sports Medicine Medicine lipids (amino acids peptides and proteins) medicine.symptom Prostaglandin E Research Article Veterinary Medicine medicine.medical_specialty 040301 veterinary sciences Immunology EP4 Receptor Prostaglandin Inflammation Microbiology Dinoprostone 03 medical and health sciences Rheumatology Tendon Injuries Internal medicine medicine Animals Horses Sports and Exercise Medicine Biology 030304 developmental biology Lipoxin business.industry lcsh:R Immunity medicine.disease Receptors Formyl Peptide Endocrinology chemistry Tendinopathy lcsh:Q Veterinary Science business Physiological Processes |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 11, p e48978 (2012) |
| Popis: | The contribution of inflammation to the pathogenesis of tendinopathy and high prevalence of re-injury is not well established, although recent evidence suggests involvement of prostaglandins. We investigated the roles of prostaglandins and inflammation-resolving mediators in naturally occurring equine tendon injury with disease stage and age. Levels of prostaglandins E(2) (PGE(2)), F(2α) (PGF(2α)), lipoxin A(4) (LXA(4)) and its receptor FPR2/ALX were analysed in extracts of normal, sub-acute and chronic injured tendons. To assess whether potential changes were associated with altered PGE(2) metabolism, microsomal prostaglandin E synthase-1 (mPGES-1), prostaglandin dehydrogenase (PGDH), COX-2 and EP(4) receptor expression were investigated. The ability of tendons to resolve inflammation was determined by assessing FPR2/ALX expression in natural injury and IL-1β stimulated tendon explants.Alterations in the profile of lipid mediators during sub-acute injury included low PGE(2) and elevated LXA(4) levels compared to normal and chronic injuries. In contrast, PGF(2α) levels remained unchanged and were three-fold lower than PGE(2). The synthetic capacity of PGE(2) as measured by the ratio of mPGES-1:PGDH was elevated in sub-acute injury, suggesting aberrations in tendon prostaglandin metabolism, whilst COX-2 and EP(4) receptor were unchanged. Paradoxically low tendon PGE(2) levels in early injury may be attributed to increased local clearance via PGDH or the class switching of lipid mediators from the prostaglandin to the lipoxin axis. PGE(2) is therefore implicated in the development of tendon inflammation and its ensuing resolution. Whilst there was no relationship between age and tendon LXA(4) levels, there was an age-associated decline in FPR2/ALX receptor expression with concurrent increased PGE(2) levels in injury. Furthermore, uninjured tendon explants from younger ( |
| Databáze: | OpenAIRE |
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