An amperometric glucose biosensor prototype fabricated by thermal inkjet printing
| Autor: | Barbara Ballarin, Alessandro Fraleoni-Morgera, C. Piana, Leonardo Setti, D. Frascaro, A. Filippini |
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| Přispěvatelé: | L.Setti, A. Fraleoni-Morgera, B. Ballarin, A. Filippini, D.Frascaro, C.Piana, Leonardo, Setti, FRALEONI MORGERA, Alessandro, Barbara, Ballarin, Alessandro, Filippini, Davide, Frascaro, Chiara, Piana |
| Rok vydání: | 2004 |
| Předmět: |
BIOELECTRONICS
Blood Glucose Materials science Hot Temperature Electronic ink Biomedical Engineering Biophysics Nanotechnology Pilot Projects Bioelectronic THERMAL INKJET PRINTING Biosensing Techniques chemistry.chemical_compound Glucose Oxidase Biopolytronics PEDOT:PSS Coated Materials Biocompatible Biopolytronic Electrochemistry Glucose oxidase Thermal inkjet printing Semipermeable membrane Computer Peripherals ELECTRONIC INK Conductive polymer biology BIOLOGICAL INK General Medicine Equipment Design Cellulose acetate Amperometry Equipment Failure Analysis Biosensors Glucose chemistry biology.protein Feasibility Studies Printing Polystyrene BIOSENSORS Aspergillus niger Biological ink Bioelectronics Biosensor Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 20(10) |
| ISSN: | 0956-5663 |
| Popis: | The prototype of an amperometric glucose biosensorwas realized by thermal inkjet printing using biological and electronicwater-based inks, containing a glucose oxidase (GOD) from Aspergillus niger and the conducting polymer blend poly(3,4-ethylenedioxythiophene/polystyrene sulfonic acid) (PEDOT/PSS), respectively. The biosensor was fabricated microdepositing PEDOT/PSS and GOD, in sequence, on ITO-glass, by a commercial inkjet printer, with the help of a commercial software. High density microdots matrices were so-realized, with a calculated resolution of about 221×221 dpi (dot per inch). By means of a rapid and easy assay it was demonstrated that no activity loss occurred upon the printing of GOD, despite of the use of a thermal printhead. The device was encapsulated in a semipermeable membrane of cellulose acetate, applied by dip-coating, in order to prevent dissolution of the enzyme and/or PEDOT/PSS in water. The preliminary response of the electrode was measured in an aqueous glucose solution in the presence of ferrocenemethanol (FeMeOH) as a mediator, and resulted linear up to 60mM in glucose. The best sensitivity value achieved was 6.43AM−1 cm−2 (447 nAM−1 U−1 cm−2). The characteristics of the device, and the possible performance improvements have been analyzed and discussed. The reported findings indicate that inkjet printing could be a viable instrument for the easy construction of a working biosensor via direct digital design using biological and conductive polymer based inks. Such an approach may be seen as an example of “biopolytronics”. |
| Databáze: | OpenAIRE |
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