Adenovirus-mediated expression of keratinocyte growth factor promotes secondary flap necrotic wound healing in an extended animal model
| Autor: | Wenquan Zhao, Tingwei Bao, Xinhua Wang, Huiming Wang, Wenyuan Zhu, Mengfei Yu, Fuyan Zhao, Li-Qin Zhu |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Necrosis Fibroblast Growth Factor 7 Gene Expression medicine.disease_cause Real-Time Polymerase Chain Reaction Surgical Flaps Adenoviridae Andrology chemistry.chemical_compound Blood serum In vivo medicine Animals Skin Wound Healing business.industry Surgery Rats medicine.anatomical_structure chemistry Models Animal Keratinocyte growth factor medicine.symptom Keratinocyte business Wound healing Plasmids |
| Zdroj: | Aesthetic plastic surgery. 37(5) |
| ISSN: | 1432-5241 |
| Popis: | No effective treatments have been found for flap necrosis. Animal models that focus on the initial flap viability are inappropriate for necrotic wound studies. Keratinocyte growth factor (KGF) promotes keratinocyte proliferation with stronger activity and fewer complications and thus may be useful for necrotic flap wound healing. Rats with modified flap necrosis were randomly divided into four groups. An adenoviral vector expressing KGF was injected subdermally in the back of the animals after necrosis began. The expression and effect of KGF was assessed by real-time polymerase chain reaction, enzyme-linked immunoassay, and transwell, and wound healing was monitored. The plasmid and adenovirus were able to express KGF and stimulate epithelial cell growth (p = 0.029). Histology showed that the necrosis healed fastest in the KGF administration group than in the control groups (p |
| Databáze: | OpenAIRE |
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