Upregulated expression of serum exosomal miR-375 and miR-1307 enhance the diagnostic power of CA125 for ovarian cancer
| Autor: | Wen Han Li, Yingying Su, Yefei Zhu, Li Sun, Ting Ting Bai, Zhi Rui Guo, Xiaoxiao Cai, Jin Chang Li |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine Lymph node metastasis Exosomes lcsh:Gynecology and obstetrics Young Adult 03 medical and health sciences Tumor Biomarkers 0302 clinical medicine Downregulation and upregulation Ovarian cancer Mir-375 microRNA Biomarkers Tumor medicine Humans lcsh:RG1-991 Aged Neoplasm Staging Aged 80 and over Ovarian Neoplasms business.industry Research Obstetrics and Gynecology Biomarker Middle Aged medicine.disease Microvesicles Up-Regulation MicroRNAs Serum exosome 030104 developmental biology Oncology Lymphatic Metastasis 030220 oncology & carcinogenesis Cancer research Biomarker (medicine) Female business |
| Zdroj: | Journal of Ovarian Research Journal of Ovarian Research, Vol 12, Iss 1, Pp 1-9 (2019) |
| ISSN: | 1757-2215 |
| DOI: | 10.1186/s13048-018-0477-x |
| Popis: | Background Ovarian cancer (OC) is associated with high mortality in gynecological oncology; this is mainly due to the low diagnosis rate. Exosomal miRNA has demonstrated potential as a tumor biomarker. We aimed to explore the diagnostic potential of serum exosomal miR-1307 and miR-375 for OC. Methods The first six candidate miRNAs were selected from the previous literature. The relative quantification of qRT-PCR was used to screen for the stability of exosomal miRNAs, followed by validation of the cohort. ROC analysis was employed to analyze the specificity and sensitivity of exosomal miRNA. Results MiR-1307 and miR-375 were confirmed stably existing in serum exosomes of OC. Moreover, miR-1307 and miR-375 were both significantly up-regulated in serum exosomes of OC compared to ovarian benign and healthy groups. The overexpressed miRNAs showed independent diagnostic power and enhanced the diagnostic accuracy of traditional biomarkers when combined with CA-125 and HE4. MiR-1307 was associated with tumor staging, and miR-375 was associated with lymph node metastasis of OC. Conclusion Our results suggest that serum exosomal miR-1307 and miR-375 could serve as potential tumor biomarkers to improve diagnostic efficiency for OC. |
| Databáze: | OpenAIRE |
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