An aerogel obtained from chemo-enzymatically oxidized fenugreek galactomannans as a versatile delivery system
| Autor: | Rossi, Bianca, Campia, Paola, Merlini, Luca, Brasca, Milena, Pastori, Nadia, Farris, Stefano, Melone, Lucio, Punta, Carlo, Galante, Yves M. |
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| Rok vydání: | 2015 |
| Předmět: |
Materials Chemistry2506 Metals and Alloys
Trigonella Galactomannans Polymers and Plastics Aerogel Delivery system Fenugreek Laccaseoxidation Lysozyme Medicine (all) Organic Chemistry 02 engineering and technology 01 natural sciences Cyclic N-Oxides Diffusion Mannans chemistry.chemical_compound Hydrolysis Materials Chemistry Organic chemistry Solubility Mechanical Phenomena Drug Carriers Aqueous solution biology 010405 organic chemistry Laccase Biomaterial Galactose Water 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Freeze Drying chemistry Muramidase 0210 nano-technology Drug carrier Gels Oxidation-Reduction |
| Zdroj: | Carbohydrate polymers 144 (2016): 353–361. doi:10.1016/j.carbpol.2016.02.007 info:cnr-pdr/source/autori:Rossi, Bianca; Campia, Paola; Merlini, Luca; Brasca, Milena; Pastori, Nadia; Farris, Stefano; Melone, Lucio; Melone, Lucio; Punta, Carlo; Galante, Yves M./titolo:An aerogel obtained from chemo-enzymatically oxidized fenugreek galactomannans as a versatile delivery system/doi:10.1016%2Fj.carbpol.2016.02.007/rivista:Carbohydrate polymers/anno:2016/pagina_da:353/pagina_a:361/intervallo_pagine:353–361/volume:144 |
| ISSN: | 1879-1344 |
| DOI: | 10.1016/j.carbpol.2016.02.007 |
| Popis: | We describe a new aerogel obtained from laccase-oxidized galactomannans of the leguminous plant fenugreek (Trigonella foenum-graecum) and suggest its potential practical use. Laccase/TEMPO oxidation of fenugreek in aqueous solution caused a viscosity increase of over 15-fold. A structured, elastic, stable hydrogel was generated, due to formation of carbonyl groups from primary OH of galactose side units and subsequent establishment of hemiacetalic bonds with available free hydroxyl groups. Upon lyophilization of this hydrogel, a water-insoluble aerogel was obtained (EOLFG), capable of uptaking aqueous or organic solvents over 20 times its own weight. The material was characterized by scanning electron microscopy, FT-IR, elemental analysis and (13)C CP-MAS NMR spectroscopy and its mechanical properties were investigated. To test the EOLFG as a delivery system, the anti-microbial enzyme lysozyme was used as model active principle. Lysozyme was added before or after formation of the aerogel, entrapped or absorbed in the gel, retained and released in active form, as proven by its hydrolytic glycosidase activity on lyophilized Micrococcus lysodeikticus cells wall peptidoglycans. This new biomaterial, composed of a chemo-enzymatically modified plant polysaccharide, might represent a versatile, biocompatible "delivery system" of active principles in food and non-food products. |
| Databáze: | OpenAIRE |
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