Immunoglobulin superfamily receptor Junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes

Autor: Larissa B. Patterson, Dae Seok Eom, David M. Parichy, Raegan R. Bostic
Rok vydání: 2021
Předmět:
Gene Expression
Medical and Health Sciences
Melanophore
Receptor tyrosine kinase
0302 clinical medicine
Morphogenesis
Developmental
Zebrafish
0303 health sciences
Pigmentation
Metamorphosis
Biological
Gene Expression Regulation
Developmental
Neural crest
Biological Sciences
Cell biology
Phenotype
Neural Crest
Iridophore
Signal Transduction
1.1 Normal biological development and functioning
Melanophores
Immunoglobulins
Biology
Pigment pattern
Article
03 medical and health sciences
Underpinning research
Genetics
Animals
Cell adhesion
Molecular Biology
Body Patterning
030304 developmental biology
Metamorphosis
Cell Biology
Zebrafish Proteins
Biological
biology.organism_classification
JAM3
Gene Expression Regulation
Mutation
Junctional Adhesion Molecule C
biology.protein
Immunoglobulin superfamily
Generic health relevance
Carrier Proteins
Cell Adhesion Molecules
030217 neurology & neurosurgery
Developmental Biology
Genetic screen
Zdroj: Dev Biol
ISSN: 0012-1606
Popis: Adhesive interactions are essential for tissue patterning and morphogenesis yet difficult to study owing to functional redundancies across genes and gene families. A useful system in which to dissect roles for cell adhesion and adhesion-dependent signaling is the pattern formed by pigment cells in skin of adult zebrafish, in which stripes represent the arrangement of neural crest derived melanophores, cells homologous to melanocytes. In a forward genetic screen for adult pattern defects, we isolated thepissarro(psr) mutant, having a variegated phenotype of spots, as well as defects in adult fin and lens. We show thatpsrcorresponds tojunctional adhesion protein 3b(jam3b) encoding a zebrafish orthologue of the two immunoglobulin-like domain receptor JAM3 (JAM-C), known for roles in adhesion and signaling in other developing tissues, and for promoting metastatic behavior of human and murine melanoma cells. We found that zebrafishjam3bis expressed post-embryonically in a variety of cells including melanophores, and thatjam3bmutants have defects in melanophore survival. Jam3b supported aggregation of cellsin vitroand was required autonomously by melanophores for an adherent phenotypein vivo. Genetic analyses further indicated both overlapping and non-overlapping functions with the related receptor, Immunoglobulin superfamily 11 (Igsf11) and Kit receptor tyrosine kinase. These findings suggest a model for Jam3b function in zebrafish melanophores and hint at the complexity of adhesive interactions underlying pattern formation.
Databáze: OpenAIRE
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