Fluorescent Markers: Proteins and Nanocrystals

Autor: Juliana Rodrigues Machado, Anielle Christine Almeida Silva, Edigar Henrique Vaz Dias, Malu Mateus Santos, Jerusa M. de Oliveira, Virmondes Rodrigues Junior, Rose Kethelyn Souza Avelino, Marcos Vinicius da Silva, Marcelo Duzzioni, Noelio O. Dantas, Kelen Talita Romão da Silva, João Paulo Santos de Carvalho, Fábio Luiz de Oliveira, Carlo José Freire de Oliveira, Eurípedes Alves da Silva Filho, Francisco Rubens Alves dos Santos, Lucas Anhezini
Rok vydání: 2021
Předmět:
DOI: 10.5772/intechopen.96675
Popis: This book chapter will comment on fluorescent reporter proteins and nanocrystals’ applicability as fluorescent markers. Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and biochemical phenomena in vivo, such as autophagy, mitophagy, and changes in the redox state of cells. Titanium dioxide (TiO2) nanocrystals (NCs) have several biological applications and emit in the ultraviolet, with doping of europium ions can be visualized in the red luminescence. Therefore, it is possible to monitor nanocrystals in biological systems using different emission channels. CdSe/CdS magic-sized quantum dots (MSQDs) show high luminescence stability in biological systems and can be bioconjugated with biological molecules. Therefore, this chapter will show exciting results of the group using fluorescent proteins and nanocrystals in biological systems.
Databáze: OpenAIRE