Evaluation of the hormonal state of columnar apple trees (Malus x domestica) based on high throughput gene expression studies
| Autor: | Stefanie Lokan, Romina Petersen, Bastienne Brauksiepe, P. Braun, Erwin R. Schmidt, Clemens Krost |
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| Rok vydání: | 2013 |
| Předmět: |
Models
Molecular Meristem Gene Expression Plant Science Biology Genes Plant Real-Time Polymerase Chain Reaction Chromosomes Plant Deep sequencing Trees Transcriptome Plant Growth Regulators Gene Expression Regulation Plant Gene expression Genetics Homeostasis Allele Gene Alleles Oligonucleotide Array Sequence Analysis Regulation of gene expression Sequence Analysis RNA Computational Biology High-Throughput Nucleotide Sequencing General Medicine Phenotype RNA Plant Malus Agronomy and Crop Science Plant Shoots |
| Zdroj: | Plant Molecular Biology. 81:211-220 |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/s11103-012-9992-0 |
| Popis: | The columnar phenotype of apple trees (Malus x domestica) is characterized by a compact growth habit with fruit spurs instead of lateral branches. These properties provide significant economic advantages by enabling high density plantings. The columnar growth results from the presence of a dominant allele of the gene Columnar (Co) located on chromosome 10 which can appear in a heterozygous (Co/co) or homozygous (Co/Co) state. Although two deep sequencing approaches could shed some light on the transcriptome of columnar shoot apical meristems (SAMs), the molecular mechanisms of columnar growth are not yet elaborated. Since the influence of phytohormones is believed to have a pivotal role in the establishment of the phenotype, we performed RNA-Seq experiments to study genes associated with hormone homeostasis and clearly affected by the presence of Co. Our results provide a molecular explanation for earlier findings on the hormonal state of columnar apple trees. Additionally, they allow hypotheses on how the columnar phenotype might develop. Furthermore, we show a statistically approved enrichment of differentially regulated genes on chromosome 10 in the course of validating RNA-Seq results using additional gene expression studies. |
| Databáze: | OpenAIRE |
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