Differential carbohydrate metabolism conducts morphogenesis in embryogenic callus of Hevea brasiliensis (Müll. Arg.)
| Autor: | Marc-Philippe Carron, Ludovic Lardet, Jean-Louis Jacob, Géraldine Blanc, Arnaud Martin |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Sucrose
Time Factors Somatic embryogenesis Physiology Métabolisme des glucides Disaccharide Plant Science Antioxidants chemistry.chemical_compound Embryogénèse somatique Morphogenesis Plant Proteins Cal food and beverages Cell Differentiation Starch Hevea brasiliensis F02 - Multiplication végétative des plantes Biochemistry Anatomie végétale Carbohydrate Metabolism Signal Transduction Teneur en glucides Carbohydrates Solution nutritive Fructose Carbohydrate metabolism Biology Culture Techniques Régénération in vitro Maltose Hexoses Peroxidase Water Carbohydrate Kinetics Glucose chemistry Callus Hevea |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/53.373.1453 |
| Popis: | Somatic embryogenesis in Hevea is stimulated when the embryogenesis induction medium contains maltose, rather than glucose, fructose, or sucrose, in equimolarity (Blanc et al., 1999). Kinetic analyses were carried out on various physiological and biochemical indicators over the 8 weeks that the induction phase then expression of somatic embryogenesis can take. Embryogenesis induction in the presence of glucose, fructose or sucrose revealed strong callus growth in the first 3-4 weeks, associated with a high intra- and extracellular hexose content, a high starch content and a substantial decline in protein synthesis. In the presence of maltose, callus growth was slow and only half that seen with sucrose. This morphogenetic behaviour is associated with a drop in endogenous hexose and starch contents, and an increase in protein synthesis in the first three weeks of culture. The induction of embryogenesis in the presence of maltose was uniform and twice as fast as with sucrose supply. At the end of culture, peroxidase activity, antioxidant and membrane protein contents increased in these calluses; these characteristics may be associated with somatic embryo organization and with the maintenance of effective membrane integrity within a nutrient environment that has become limiting. These new results tally with data in the literature on the roles of sugars, and provide some precise information with regard to the 'carbohydrate deficit' hypothesis usually put forward to explain maltose action. An analysis of these results led to the hypothesis that regulation of endogenous hexose contents at a low level, through slow maltose hydrolysis, was a key element of the biochemical signal leading this callus towards somatic embryogenesis. |
| Databáze: | OpenAIRE |
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