Prevalence of interferon type I signature in CD14 monocytes of patients with Sjögren's syndrome and association with disease activity and BAFF gene expression
Autor: | Virgil A. S. H. Dalm, Manon E Wildenberg, Zana Brkic, Marjan A. Versnel, Paul L A van Daele, Hemmo A. Drexhage, Cornelia G van Helden-Meeuwsen, Joop P. van de Merwe, Naomi I Maria, Wouter Beumer |
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Přispěvatelé: | Immunology, Internal Medicine, AII - Amsterdam institute for Infection and Immunity, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Gastroenterology and Hepatology |
Rok vydání: | 2012 |
Předmět: |
Adult
Male CD14 Lymphocyte Immunology Lipopolysaccharide Receptors Gene Expression Real-Time Polymerase Chain Reaction Monocytes General Biochemistry Genetics and Molecular Biology stomatognathic system Rheumatology Interferon B-Cell Activating Factor Prevalence medicine Humans Immunology and Allergy Rheumatoid factor RNA Messenger B-cell activating factor Aged business.industry Monocyte Autoantibody Middle Aged Clinical and Epidemiological Research Up-Regulation stomatognathic diseases Sjogren's Syndrome medicine.anatomical_structure Interferon Type I Female business Biomarkers Interferon type I medicine.drug |
Zdroj: | Annals of the Rheumatic Diseases, 72(5), 728-735. BMJ Publishing Group Annals of the rheumatic diseases, 72(5), 728-735. BMJ Publishing Group Annals of the Rheumatic Diseases |
ISSN: | 1468-2060 0003-4967 |
DOI: | 10.1136/annrheumdis-2012-201381 |
Popis: | Objective To determine the prevalence of upregulation of interferon (IFN) type I inducible genes, the so called ‘IFN type I signature’, in CD14 monocytes in 69 patients with primary Sjögren's syndrome (pSS) and 44 healthy controls (HC) and correlate it with disease manifestations and expression of B cell activating factor (BAFF). Methods Expression of IFI44L, IFI44, IFIT3, LY6E and MX1 was measured using real time quantitative PCR in monocytes. Expression values were used to calculate IFN type I scores for each subject. pSS patients positive for the IFN type I signature (IFN score≥10) and patients negative for the signature (IFN score |
Databáze: | OpenAIRE |
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