Isothermal microcalorimetry measures UCP1-mediated thermogenesis in mature brite adipocytes

Autor: Ez-Zoubir Amri, Alice Åslund, Carina Halleskog, Muhammad Hamza Bokhari, Robert I. Csikasz, Irina G. Shabalina, Eva Casadesús Rendos, Nathalie Boulet, Tore Bengtsson, Jasper M. A. de Jong
Přispěvatelé: Amri, Ez-Zoubir, Stockholm University, Institut des Maladies Métaboliques et Casdiovasculaires (UPS/Inserm U1297 - I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Yale School of Medicine [New Haven, Connecticut] (YSM), Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), 3.Université Côte D'Azur, CNRS, Inserm, iBV, Nice, France, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Isothermal microcalorimetry
Male
QH301-705.5
[SDV]Life Sciences [q-bio]
Medicine (miscellaneous)
Adrenergic
Adipose tissue
[SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biophysics
Stimulation
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Calorimetry
General Biochemistry
Genetics and Molecular Biology
Article
03 medical and health sciences
Mice
0302 clinical medicine
[SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Animals
Adipocytes
Beige
Biology (General)
Uncoupling Protein 1
030304 developmental biology
[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
0303 health sciences
Chemistry
Biological techniques
Wild type
Thermogenesis
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Thermogenin
Cell biology
[SDV] Life Sciences [q-bio]
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biophysics
Brite Adipocytes
General Agricultural and Biological Sciences
Fat metabolism
030217 neurology & neurosurgery
Zdroj: Communications Biology
Communications Biology, 2021, 4 (1), pp.1108. ⟨10.1038/s42003-021-02639-4⟩
Communications Biology, Nature Publishing Group, 2021, 4 (1), ⟨10.1038/s42003-021-02639-4⟩
Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
Communications Biology, 2021, 4 (1), ⟨10.1038/s42003-021-02639-4⟩
ISSN: 2399-3642
Popis: The activation of thermogenesis in adipose tissue has emerged as an important target for the development of novel anti-obesity therapies. Using multi-well isothermal microcalorimetry, we have demonstrated that mature murine brown and brite adipocytes produce quantifiable heat upon β3-AR stimulation, independently of any anaerobic mechanisms. Additionally, in brite adipocytes lacking UCP1 protein, β3-AR stimulation still induces heat production, albeit to a much lower extent than in their wildtype counterparts, suggesting that UCP1 is an essential component of adrenergic induced thermogenesis in murine brite adipocytes exvivo. Similarly, we could observe an increase in heat production in human-derived adipocytes (hMADS) upon β-AR stimulation. Collectively, these results establish the use of isothermal microcalorimetry as a sensitive and accurate technique for measuring thermogenic responses in intact mature brite adipocytes from murine and human origin.
Bokhari et al. demonstrate mature murine brown and brite adipocytes produce quantifiable heat with β3-AR stimulation. They indicate that the essential component of this mechanism is UCP1 by using adipocytes lacking the protein.
Databáze: OpenAIRE
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