Determination of 2-methylimidazole, 4-methylimidazole and 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole in caramel colours and cola using LC/MS/MS
| Autor: | Julia Schrank, Dirk W. Lachenmeier, Fanette Laplagne, Rüdiger Schneider, Claudia Schlee, Mariya Markova |
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| Rok vydání: | 2012 |
| Předmět: |
Detection limit
Chromatography biology Chemistry Clinical Biochemistry Carbohydrates Imidazoles Food Coloring Agents Reproducibility of Results Carbonated Beverages Cell Biology General Medicine 2-Methylimidazole biology.organism_classification Biochemistry Sensitivity and Specificity Cola (plant) Analytical Chemistry chemistry.chemical_compound Tandem Mass Spectrometry Lc ms ms Imidazole 4-Methylimidazole Sample preparation Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 927 |
| ISSN: | 1873-376X |
| Popis: | Substituted imidazoles recently came under scrutiny as they may be indirectly introduced into cola beverages via the use of class IV (E150d) caramel colours and may pose health hazards. A LC/MS/MS method was developed for determining 2- and 4-methylimidazole (2-MI, 4-MI) and 2-acetyl-4-(1,2,3,4)-tetrahydroxybutylimidazole (THI) in beverages and caramel colours. The method is very rapid and easy to conduct as it requires only dilution in eluent for sample preparation. For 4-MI, the recovery was between 94 and 102% for spiked cola samples. The limit of detection was 2μg/L in the measuring solution (corresponding to 40μg/L for cola samples diluted 1:20 during sample preparation). 97 cola samples and 13 caramel colours from Germany and France were analysed. From the 3 analytes, only 4-MI was found in the samples with very varying concentrations (non quantifiable traces to 0.6mg/L in colas and 175-658mg/kg in E150d). The exposure for cola drinkers in worst case scenarios is estimated to be 2-5μg/kg bodyweight/day, which is judged as being only a low risk for public health. |
| Databáze: | OpenAIRE |
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