Traditional Chinese Medicine Extract from Huaier Increases the Expression of Duffy Antigen Receptor for Chemokines and Reduces the Expression of Its Ligands

Autor: Qianjun Chen, Shaowen Zhong, Fengfeng Xie, Wenxia Li, Xie Wanjun, Hui Peng, Zhiyu Wang, Ying Chen, Yanlan Wu
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Cancer Research
Chemokine
Chemokine CXCL1
Gene Expression
Metastasis
0302 clinical medicine
Medicine
Medicine
Chinese Traditional
Receptor
Chemokine CCL2
RC254-282
Trametes
biology
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
General Medicine
Middle Aged
Metastatic breast cancer
Blot
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
Matrix Metalloproteinase 2
Molecular Medicine
Immunohistochemistry
Female
Research Article
Adult
Article Subject
Blotting
Western
Breast Neoplasms
Enzyme-Linked Immunosorbent Assay
Receptors
Cell Surface
Complex Mixtures
Real-Time Polymerase Chain Reaction
Pathology and Forensic Medicine
03 medical and health sciences
Breast cancer
Humans
Aged
QH573-671
business.industry
Interleukin-8
Cell Biology
medicine.disease
030104 developmental biology
Cancer research
biology.protein
Duffy Blood-Group System
business
Cytology
Zdroj: Analytical Cellular Pathology, Vol 2018 (2018)
Analytical Cellular Pathology (Amsterdam)
ISSN: 2210-7185
2210-7177
Popis: Aims. The aim of the present study is to investigate whether the aqueous extract from Huaier, a traditional Chinese medicine (TCM), can affect the expression of Duffy antigen receptor for chemokines (DARC) and its ligands. Moreover, we compare the status of DARC in primary and metastatic breast cancer tissues from the same patient.Methods. Immunohistochemistry was used to detect the expression of DARC in primary and metastatic focuses in 30 patients with breast cancer. The effect of Huaier aqueous extract on the expression of DARC and its ligands was investigated by quantitative real-time polymerase chain reaction, Western blotting, and enzyme-linked immunosorbent assay.Results. The expression score of DARC in primary focuses was significantly higher than that in metastatic focuses, while changes of ER, PR, and HER2 receptors were not significantly different between primary and metastatic focuses. Huaier aqueous extract promoted the expression of DARC and reduced the secretion of CC chemokine ligand 2 (CCL-2), CXC chemokine ligand 8 (CXCL-8, IL-8), matrix metalloproteinase 2 (MMP-2), and CXC chemokine ligand 1 (CXCL-1).Conclusion. The present study demonstrates that difference in expression level of DARC between primary and metastatic focuses of breast cancer was significant, while differences in expression of ER, PR, and HER2 between primary and metastatic focuses were not significant. DARC may play a negative role in the metastasis of breast cancer. Traditional Chinese medicine extract from Huaier can increase DARC expression and reduce the expression of its ligands such as CCL-2, IL-8, MMP-2, and CXCL-1.
Databáze: OpenAIRE
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