Protection against exonuclease III digestion A new way to investigate protein-DNA interactions
| Autor: | R. Wayne Davies, M.L. Kotewicz, P.H. Schreier |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Exonucleases
Biophysics Plasma protein binding Biochemistry DNA-binding protein chemistry.chemical_compound Structural Biology Transcription (biology) Genetics A-DNA Binding site Molecular Biology Exonuclease III Binding Sites Deoxyribonucleases biology DNA Helicases Proteins Cell Biology DNA Bacteriophage lambda Integrase Exodeoxyribonucleases chemistry biology.protein Protein Binding |
| Zdroj: | FEBS Letters. (1):159-163 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(80)81333-0 |
| Popis: | Interactions between proteins and DNA molecules form the basis of many biological control mechanisms and of the central processes of molecular biology, replication and transcription. Virtually everything that happens to DNA molecules involves some protein binding to it in a particular way before beginning to act. Control proteins and proteins involved in sitespecific recombination do not just recognise general features of a DNA molecule but bind specifically to a particular DNA sequence. In such cases the first step in an investigation of the DNA-protein interaction is to locate the binding site. Clearly every tool that helps us study these interactions is useful, and we report here another way of approaching this problem, which we have used in our studies on the binding of h integrase to the phage attachment site. A substrate for exonuclease III digestion is synthesised enzymatitally in vitro so that the double-stranded section contains the presumed binding region, and the results of digesting this substrate in the presence or absence of the DNA binding protein are compared. |
| Databáze: | OpenAIRE |
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