The first in-depth exploration of the genome of the engineered bacterium, Gluconobacter thailandicus
| Autor: | Yupeng Liu, Chenyi Liu, Pengpai Zhang, Afsana Ali, Xiaoxiao Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Glycerol Gluconobacter Citric Acid Cycle Biomedical Engineering PQQ Cofactor Dihydroxyacetone Bioengineering Gluconobacter thailandicus 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Pyrroloquinoline quinone 010608 biotechnology Drug Discovery Gluconobacter oxydans Phylogeny 030304 developmental biology 0303 health sciences Chemistry Process Chemistry and Technology food and beverages General Medicine NAD Metabolic pathway Biochemistry Glycerol dehydrogenase Molecular Medicine Microorganisms Genetically-Modified Genetic Engineering Genome Bacterial NADP Biotechnology Sugar Alcohol Dehydrogenases |
| Zdroj: | Biotechnology and applied biochemistryREFERENCES. 69(3) |
| ISSN: | 1470-8744 |
| Popis: | Glycerol is an abundant byproduct of biodiesel production that has significant industrial value and can be converted into dihydroxyacetone (DHA). DHA is widely used for the production of various chemicals, pharmaceuticals, and food additives. Gluconobacter can convert glycerol to DHA through two different pathways, including membrane-bound dehydrogenases with pyrroloquinoline quinone (PQQ) and NAD(P)+ -dependent enzymes. Previous work has indicated that membrane-bound dehydrogenases are present in Gluconobacter oxydans and Gluconobacter frateurii, but the metabolic mechanism of Gluconobacter thailandicus's glycerol conversion is still not clear. Through in-depth analysis of the G. thailandicus genome and annotation of its metabolic pathways, we revealed the existence of both PQQ and NAD(P)+ -dependent enzymes in G. thailandicus. In addition, this study provides important information related to the tricarboxylic acid cycle, glycerol dehydrogenase level, and phylogenetic relationships of this important species. |
| Databáze: | OpenAIRE |
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