Reaction of PerR with Molecular Oxygen May Assist H2O2 Sensing in Anaerobes
| Autor: | Eric Gouré, Ramakrishnan Sethu, Luca Signor, Martin Clémancey, Jean-Marc Latour, Victor Duarte, Christelle Caux-Thang |
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| Přispěvatelé: | Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) |
| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Staphylococcus aureus 030106 microbiology Microbial metabolism Regulator medicine.disease_cause Bioinformatics Biochemistry Peroxide Bacteria Anaerobic 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins medicine [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology chemistry.chemical_classification biology Pathogenic bacteria Hydrogen Peroxide General Medicine biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Oxygen Repressor Proteins Enzyme chemistry Molecular Medicine Oxidation-Reduction DNA Bacteria Bacillus subtilis |
| Zdroj: | ACS Chemical Biology ACS Chemical Biology, American Chemical Society, 2016, 11 (5), pp.1438-1444. ⟨10.1021/acschembio.5b01054⟩ ACS Chemical Biology, 2016, 11 (5), pp.1438-1444. ⟨10.1021/acschembio.5b01054⟩ |
| ISSN: | 1554-8937 1554-8929 |
| Popis: | International audience; PerR is the peroxide resistance regulator found in several pathogenic bacteria and governs their resistance to peroxide stress by inducing enzymes that destroy peroxides. However, it has recently been implicated as a key component of the aerotolerance in several facultative or strict anaerobes, including the highly pathogenic Staphylococcus aureus. By combining (18)O labeling studies to ESI- and MALDI-TOF MS detection and EMSA experiments, we demonstrate that the active form of PerR reacts with dioxygen, which leads ultimately to disruption of the PerR/DNA complex and is thus physiologically meaningful. Moreover, we show that the presence of O2 assists PerR sensing of H2O2, another feature likely to be important for anaerobic organisms. These results allow one to envisage different scenarios for the response of anaerobes to air exposure. |
| Databáze: | OpenAIRE |
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