Interaction between βC1 of satellite and coat protein of Chili leaf curl virus plays a crucial role in suppression of host RNA silencing
| Autor: | Anurag Kumar Sahu, Neeti Sanan-Mishra |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
biology
Nicotiana tabacum Two-hybrid screening fungi Green Fluorescent Proteins food and beverages General Medicine biology.organism_classification Subcellular localization Applied Microbiology and Biotechnology Green fluorescent protein Cell biology RNA silencing Begomovirus Tobacco Gene silencing Satellite (biology) RNA Interference Gene Silencing Nuclear localization sequence Biotechnology Plant Diseases |
| Zdroj: | Applied microbiology and biotechnologyReferences. 105(21-22) |
| ISSN: | 1432-0614 |
| Popis: | The monopartite Chili leaf curl virus (ChiLCV) and its β-satellite (ChiLCB) have been found to co-exist in infected plants. The ability of βC1 protein to suppress RNA silencing was investigated using an in-house developed in-planta reversal of silencing assay, using Nicotiana tabacum lines harboring green fluorescent protein (GFP) silenced by short hairpin GFP (ShGFP). Transient expression of recombinant βC1 complemented and increased the suppressor activity of ChiLCV coat protein (CP), and this was confirmed by molecular analysis. In silico analysis followed by a yeast two-hybrid screen-identified ChiLCV-CP as the interacting partner of the ChiLCB-βC1 protein. Subcellular localization through confocal analysis revealed that when βC1 and ChiLCV-CP were co-present, the fluorescence was localized in the cytoplasm indicating that nuclear localization of both proteins was obstructed. The cytoplasmic compartmentalization of the two viral suppressors of RNA silencing may be responsible for the enhanced suppression of the host gene silencing. This study presents evidence on the interaction of ChiLCV-CP and βC1 proteins and indicates that ChiLCB may support the ChiLCV in overcoming host gene silencing to cause Chili leaf curl disease. KEY POINTS: • CP of ChiLCV and βC1 of ChiLCB contain RNA silencing suppression activity • The RNA silencing suppression activity of ChiLCB-βC1 complements that of ChiLCV-CP • There is a direct interaction between ChiLCB-βC1 and ChiLCV-CP. |
| Databáze: | OpenAIRE |
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