Deoxycholate-induced colitis is markedly attenuated in Nos2 knockout mice in association with modulation of gene expression profiles
| Autor: | Eugene W. Gerner, Katerina Dvorak, Claire M. Payne, Harris Bernstein, Hana Holubec, Jose L. Padilla-Torres, David G. Besselsen, Natalia A. Ignatenko, Karen A. Blohm-Mangone, Carol Bernstein, Barbora Dvorakova, Harinder S. Garewal |
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| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_specialty
Physiology medicine.drug_class Ratón Detergents Inflammation Mice Inbred Strains Inflammatory bowel disease Tight Junctions Mice Internal medicine parasitic diseases Gene expression medicine Animals Colitis Intestinal Mucosa Oligonucleotide Array Sequence Analysis Bile acid business.industry Gene Expression Profiling Gastroenterology Membrane Proteins respiratory system medicine.disease Ulcerative colitis Immunohistochemistry Disease Models Animal Oxidative Stress Endocrinology Immunology Knockout mouse Dietary Supplements Disease Progression medicine.symptom Nitric Oxide Synthase business DNA Damage Deoxycholic Acid |
| Zdroj: | Digestive diseases and sciences. 52(3) |
| ISSN: | 0163-2116 |
| Popis: | Nos2 knockout mice were compared to wild-type mice for susceptibility to colitis in response to a diet supplemented with deoxycholate, a bile acid increased in the colon of individuals on a high-fat diet. Wild-type mice fed a fat-related diet, supplemented with 0.2% DOC, develop colonic inflammation associated with increases in nitrosative stress, proliferation, oxidative DNA/RNA damage, and angiogenesis, as well as altered expression of numerous genes. However, Nos2 knockout mice fed a diet supplemented with deoxycholate were resistant to these alterations. In particular, 35 genes were identified whose expression was significantly altered at the mRNA level in deoxycholate-fed Nos2(+/+) mice but not in deoxycholate-fed Nos2(–/–) mice. Some of these alterations in NOS2-dependent gene expression correspond to those reported in human inflammatory bowel disease. Overall, our results indicate that NOS2 expression is necessary for the development of deoxycholate-induced colitis in mice, a unique dietary-related model of colitis. |
| Databáze: | OpenAIRE |
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