Simultaneous measurements of changes in sarcoplasmic reticulum and cytosolic [Ca2+] in rat uterine smooth muscle cells
| Autor: | David A. Eisner, Susan Wray, A. V. Shmigol |
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| Rok vydání: | 2001 |
| Předmět: |
Agonist
medicine.medical_specialty Carbachol Thapsigargin Physiology medicine.drug_class Biology Uterine Contraction chemistry.chemical_compound Cytosol Internal medicine medicine Extracellular Animals Magnesium Rats Wistar Calcium metabolism Rapid Report Endoplasmic reticulum Osmolar Concentration Uterus Reproducibility of Results Muscle Smooth Depolarization Rats Sarcoplasmic Reticulum Endocrinology chemistry Biophysics Calcium Female Artifacts medicine.drug |
| Zdroj: | The Journal of Physiology. 531:707-713 |
| ISSN: | 1469-7793 0022-3751 |
| DOI: | 10.1111/j.1469-7793.2001.0707h.x |
| Popis: | 1. The role of the sarcoplasmic reticulum (SR) was investigated in spontaneous and agonist-induced uterine Ca2+ transients, by combining low- (mag-fluo-4) and high-affinity (fura-2) indicators to measure intraluminal SR ([Ca2+]L) and cytosolic ([Ca2+]i) calcium concentration, simultaneously, in single smooth muscle cells from pregnant rat uterus. 2. Carbachol or ATP, in the absence of extracellular Ca2+, decreased [Ca2+]L and increased [Ca2+]i. Although some replenishment (around 50 %) occurred in its absence, extracellular Ca2+ was required for full replenishment of the SR Ca2+. 3. In 4/15 cells, ATP evoked oscillations of [Ca2+]i. These were accompanied by successive release and re-uptake of SR Ca2+. Inhibition of the SR Ca2+-ATPase with thapsigargin abolished the oscillations and luminal changes. 4. Spontaneous [Ca2+]i transients produced no detectable changes in [Ca2+]L. The larger [Ca2+]i transients evoked by high-K+ depolarisation increased [Ca2+]L. Spontaneous activity was inhibited when [Ca2+]L was increased. 5. These data show that it is possible to simultaneously measure SR and cytosolic [Ca2+], and to investigate their response to agonist application and spontaneous activity. |
| Databáze: | OpenAIRE |
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